Anthocyanin profile and effect at glaucoma Vaccinium arctostaphylos and Vaccinium myrtillus fruits
- Authors: Ibaeva K.A.1, Shamilov A.A.1, Tashlitsky V.N.2, Pozdnyakov D.I.1
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Affiliations:
- Volgograd State Medical University Ministry of Health of Russian Federation
- M.V. Lomonosov Moscow State University
- Issue: Vol 28, No 9 (2025)
- Pages: 70-79
- Section: Problems of experimental biology and medicine
- URL: https://ogarev-online.ru/1560-9596/article/view/310641
- DOI: https://doi.org/10.29296/25877313-2025-09-10
- ID: 310641
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Abstract
Introduction. We may use the sources of anthocyanins for prevention of glaucoma in the medicine practice. Pharmacopoeia source of anthocyanins is fruits of bilberry. Related species such as Vaccinium arctostaphyllos may be additional source of anthocyanins along with Vaccinium myrtillus.
Aim – the comparative investigation of anthocyanin profile of Vaccinium arctostaphylos and V. myrtillus fruits and its effects at the progress of glaucoma.
Material and methods. We prepared two types of extracts: without and with addition of hydrochloric acid. We got anthocyanins profile by high-performance liquid chromatography with UV-detector. Glaucoma model was prepared by injection of polystyrene microballons in anterior chamber of rats with subsequent measurement of activity of succinate dehydrogenase and concentration of thiobarbituric acid reactive products (TBARS) in the rat’s brain tissues.
Results. Water-alcohol extracts with addition of hydrochloric acid from bilberry fruits contain three anthocyanins: delphinidin, cyanidin, mixture peonidin and malvidin. Cyanidin is the major components in the both types of fruits. Water-alcohol extracts without hydrochloric acid content nine substances: delphinidin 3-galactoside, delphinidin 3-glucoside, cyanidin 3-galactoside in mixture with delphinidin 3-arabinoside, cyanidin 3-glucoside, petunidin 3-glucoside, peonidin 3-glucoside, malvidin 3-glucoside. Malvidin 3-glucoside and delphinidin 3-glucoside are the main glycosides in the V. arctostaphylos fruits, cyaniding 3-glucoside and glycosides of delphinidin – in the V. myrtillus fruits. Water-alcohol extracts with addition of hydrochloric acid had more effective as neuroprotective agent by increasing concentration of succinate dehydrogenase and decreasing amount of TBARS in the brain.
Conclusion. Extracts from bilberry fruits have neuroprotective effect by presence of total amount of anthocyanins and may be used for prevention of glaucoma.
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##article.viewOnOriginalSite##About the authors
Kh. A. Ibaeva
Volgograd State Medical University Ministry of Health of Russian Federation
Author for correspondence.
Email: ibaeva.hadizhat@mail.ru
ORCID iD: 0009-0002-7900-4559
SPIN-code: 6678-9776
Post-graduate Student, Department of Pharmacognosy, Botany and Technology of Phytopreparations; Pyatigorsk Medical-Pharmaceutical Institute
Russian Federation, Kalinina Avenue, 11, Pyatigorsk,357532A. A. Shamilov
Volgograd State Medical University Ministry of Health of Russian Federation
Email: shamilovxii@yandex.ru
ORCID iD: 0000-0002-6730-9518
SPIN-code: 5834-3249
Dr.Sc. (Pharm.), Associate Professor, Professor of Department of Pharmacognosy, Botany and Technology of Phytopreparations; Pyatigorsk Medical-Pharmaceutical Institute
Russian Federation, Kalinina Avenue, 11, Pyatigorsk,357532V. N. Tashlitsky
M.V. Lomonosov Moscow State University
Email: tashlitsky@belozersky.msu.ru
ORCID iD: 0000-0003-4100-2419
SPIN-code: 2590-8903
Ph.D. (Chem.), Senior Research Scientist, Department of Chemistry of Natural Compounds, Chemical Faculty
Russian Federation, Leninskie Gory, GSP-1, building 40, Moscow, 119991D. I. Pozdnyakov
Volgograd State Medical University Ministry of Health of Russian Federation
Email: pozdniackow.dmitry@yandex.ru
ORCID iD: 0000-0003-0889-7855
SPIN-code: 6764-0279
Ph.D. (Pharm.), Head of Department of Pharmacology with course of Clinical Pharmacology; Pyatigorsk Medical-Pharmaceutical Institute
Russian Federation, Kalinina Avenue, 11, Pyatigorsk,357532References
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