Changes in the elemental composition of the chyme and metabolic processes in the rumen when exogenous enzymes are used in feeding bulls

Introduction. Coordination of the processes of scar digestion and metabolism in ruminants is a prerequisite for achieving high efficiency in the use of feed nutrients, improving product quality and profitability of production.

The purpose of this study was to evaluate whether the inclusion of the exogenous lipase enzyme in the diet of bullocks in feedlots has a positive effect on physiology, fermentation in the rumen, digestibility and the profile of fatty acids in the rumen.

Material and methods. In the experiment, 8 animals were used, divided into 2 groups. the bulls of the control group received the basic diet (RR), the experimental group also received RR with the inclusion of the lipase enzyme at a dosage of 25 g / head / day. The level of volatile fatty acids (VFA) in the contents of the scar was determined by gas chromatography on a gas chromatograph "Crystallux-4000M", determination of nitrogen forms according to GOST 26180-84. The elemental composition of biosubstrates was studied using atomic emission and mass spectrometry (NPP-ISP and MS-ISP) in the IC of the Central Research Center of the FNC BST RAS.

Results. The study of the ratio of LVH in the scar fluid showed that in the experimental group of animals the content of acetic acids decreased by 18.35%, propionic acid – 15.29%, butyric acid – 34.28%, valerian acid – 11.11%, while caproic acid was not found in the experimental group. Nitrogen metabolism in the body showed that the level of total nitrogen in the scar fluid increased by 64.71%, non–protein 53.66%, protein 66.22%, ammonia 78.57% (p <0.05), respectively. Analysis of the biomass of bacteria and protozoa showed that during the experiment, these indicators were higher in the experimental group of animals by 17.07% and 22.22%, respectively, compared with the control. The trace element composition of the scar fluid revealed differences in the mechanism of action of the lipase enzyme on the metabolism of chemicals. There was an increase in the concentration of chemical elements in the experimental group of bulls Ca – by 11.51%, P –17.72%, K – 20.34% (p≤0.05), Fe – 21.31%, Zn – 10.14%, Cu – 23.53%, Se – 39.13%. Against the background of a decrease in Ni – 11.44%, Ba – 14.67%, B – 9.00%, I – 33.33%, Al – 15.71 and Sr – 15.85% (p<0.05).

Conclusion. Thus, the study of microbial communities of the LJ rumen, primarily of cattle, is very relevant due to the possibility of rapid diagnosis and prevention of many pathologies of the digestive system associated with improper feeding, which, as a rule, is accompanied by the development of pathogenic and opportunistic microorganisms.