Change of spontaneous oxidative modifications of spermоplasm's proteins in patients with varicocele and asthenozoospermia
- Authors: Ishtulin A.F.1, Korotkova N.V.1, Matveeva I.V.1, Ishtulina S.L.2, Karpov E.I.3, Pobochnaya O.V.4
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Affiliations:
- Ryazan State Medical University named after I. P. Pavlov
- Ryazan State Medical University named after I.P. Pavlov
- GBU RO "City Clinical Polyclinic No. 6"
- GBU RO "OKKVD"
- Issue: Vol 26, No 1 (2023)
- Pages: 30-34
- Section: Biological chemistry
- URL: https://ogarev-online.ru/1560-9596/article/view/250512
- DOI: https://doi.org/10.29296/25877313-2023-01-06
- ID: 250512
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Abstract
Oxidative stress plays one of the leading roles in the pathogenesis of varicocele, which affects the quantity and quality of seminal fluid. The research of ejaculate are currently based mainly on chemical-microscopic methods. Biochemical parameters of sperm are determined in insufficient volume, therefore, the introduction of new biochemical markers in order to diagnose a decrease in male fertility is an urgent issue. Oxidatively modified proteins can be considered as a marker of decreased fertility in men with varicocele.
The aim of the work was to research the carbonylation of spermоplasm proteins in patients with varicocele II and III degrees with accompanying asthenozoospermia in the anamnesis.
Material and methods. The study shows results of 60 men medical examination. The patients weredivided into two groups. The first group consisted of 30 men diagnosed with grade II and III varicocele with accompanying asthenozoospermia. The second group consisted of 30 patients with normozoospermia and without impaired reproductive function. The material for the study was spermoplasm. Spermoplasm was obtained by centrifugation of sperm samples for 10 minutes at a speed of 1000 rpm. Chemical microscopic examination of the ejaculate was carried out on a sperm analyzer (AFS-500-2, Russia) and light microscopy (microscope MS 100 X, Austria). Determination of protein concentration in spermoplasm was carried out by biuretic method with commercial kits of the company (Mindrey, China) on the biochemical analyzer Mindrey BS 120 (China). Determination of oxidative modification of proteins in spermoplasm was carried out by the method of R.L. Levine in modification by E.E. Dubinina.
Results. There was a 2-fold increase in ADNPhG at λ 356, and λ 430 by 3.2 times in patients of the first group. It was also noted that the increase in КDNPhG at λ 370 increased by 2 times, and λ 530 by 3.9 times in the spermoplasm, in contrast to patients of the control group. It was also noted that the increase in KDNPhG at λ 370 increased by 2 times, and λ 530 by 3.9 times in the spermoplasm, in contrast to patients in the control group.
Conclusion. An increase in oxidatively modified proteins in patients with varicocele may be associated with an increase in oxidative processes, a decrease in the antioxidant defense of the body and damage to the DNA of spermatozoa, which leads to a violation of its functions.
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##article.viewOnOriginalSite##About the authors
A. F. Ishtulin
Ryazan State Medical University named after I. P. Pavlov
Author for correspondence.
Email: a.ishtulin@yandex.ru
ORCID iD: 0000-0001-9750-1642
Assistant, the Department of Biological Chemistry with Course of Clinical Laboratory Diagnostics, the Faculty of Additional Professional Education
Russian Federation, RyazanN. V. Korotkova
Ryazan State Medical University named after I. P. Pavlov
Email: fnv8@yandex.ru
ORCID iD: 0000-0001-7974-2450
Ph.D. (Med.), Associate Professor, the Department of Biological Chemistry with Course of Clinical Laboratory Diagnostics, the Faculty of Additional Professional Education; Senior Research Scientist, the Central Research Laboratory
Russian Federation, RyazanI. V. Matveeva
Ryazan State Medical University named after I. P. Pavlov
Email: i.matveeva@rzgmu.ru
ORCID iD: 0000-0001-9475-8127
Ph.D. (Med.), Associate Professor, Head of the Department of Biological Chemistry with the Course of Clinical Laboratory Diagnostics, the Faculty of Additional Professional Education
Russian Federation, RyazanS. L. Ishtulina
Ryazan State Medical University named after I.P. Pavlov
Email: s.ishtulina@yanedex.ru
ORCID iD: 0000-0001-6095-0030
Assistant, the Department of Biological Chemistry with Course of Clinical Laboratory Diagnostics, the Faculty of Additional Professional Education
Russian Federation, RyazanE. I. Karpov
GBU RO "City Clinical Polyclinic No. 6"
Email: a.ishtulin@yandex.ru
Ph.D. (Med.), Urologist of the Highest Category
Russian Federation, RyazanO. V. Pobochnaya
GBU RO "OKKVD"
Email: a.ishtulin@yandex.ru
Doctor of the KLD, Laboratory for the Diagnosis of HIV Infection and AIDS-indicator Diseases
Russian Federation, RyazanReferences
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