Oxycinnamic acids as NOX4 inhibitors in Alzheimer's disease therapy. An experimental study
- Authors: Pozdnyakov D.I.1
-
Affiliations:
- Pyatigorsk Medical and Pharmaceutical Institute
- Issue: Vol 26, No 6 (2023)
- Pages: 45-49
- Section: Problems of experimental biology and medicine
- URL: https://ogarev-online.ru/1560-9596/article/view/249960
- DOI: https://doi.org/10.29296/25877313-2023-06-07
- ID: 249960
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Abstract
Relevance. Alzheimer's disease is a terminal form of dementia with a complex pathogenesis in which NOX-dependent oxidative stress plays an extremely important role.
Aim of the study. To evaluate the influence of oxycinnamic acids on the alteration of NOX4 activity in brain tissue of animals with experimental Alzheimer's disease.
Material and methods. Alzheimer's disease was modeled in Wistar rats by injection of β-amyloid aggregates with amino acid sequence 1-42 into hippocampal tissue (CA1 segment). Oxycinnamic acids: caffeic acid, coumaric acid, ferulic acid and synapic acid were administered at a dose of 100 mg/kg, orally for 60 days after the Alzheimer's disease model. Ethylmethylhydroxypyridine succinate was used as a reference drug in a similar dosage and dosing mode. Changes in the concentration of active NOX4 isophrome, hydrogen peroxide as well as β-amyloid in brain tissue of rats were assessed after the indicated time period.
Results. This study showed that the analyzed oxycinnamic acids were comparable with each other and the referent. Thus, a statistically significant (p<0.05) decrease of NOX4, hydrogen peroxide and β-amyloid concentrations was observed against the background of the studied substances application relative to the untreated animals. Correlation analysis showed that there was a strong correlation (r= 0.96968) between changes in β-amyloid and NOX4 content. Also a strong correlation relationship is observed in the case of analysis of changes in β-amyloid and hydrogen peroxide concentration (r=0,97060).
Conclusion. On the basis of the obtained data, it may be assumed that the use of oxycinnamic acids decrease the activity of NOX4, resulting in reduced formation of peroxides and, as a consequence, oxidative stress. At the same time, reduction of oxidative processes intensity leads to decrease of β-amyloid content in brain tissue.
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##article.viewOnOriginalSite##About the authors
D. I. Pozdnyakov
Pyatigorsk Medical and Pharmaceutical Institute
Author for correspondence.
Email: pozdniackow.dmitry@yandex.ru
Ph.D. (Pharm.), Head of Living System Laboratory, Associate Professor, Department of Pharmacology with Clinical Pharmacology Course
Russian Federation, PyatigorskReferences
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