Component composition of essential oil Thymus pulchellus S. A. MEY., introduced under Dagestan conditions
- Authors: Vagabova F.A.1, Aliev A.M.1
-
Affiliations:
- Dagestan Federal Research Center of the Russian Academy of Sciences
- Issue: Vol 27, No 1 (2024)
- Pages: 55-61
- Section: Pharmaceutical chemistry
- URL: https://ogarev-online.ru/1560-9596/article/view/249789
- DOI: https://doi.org/10.29296/25877313-2024-01-07
- ID: 249789
Cite item
Open Access
Access granted
Subscription Access
Abstract
The purpose of the study is to study the component composition of the essential oil in the aerial part of the endemic Krasnodar specimen Thymus pulchellus S. A. Mey., transplanted to the Tsudakhar experimental base of the Mountain Botanical Garden of the Dagestan Federal Research Center of the Russian Academy of Sciences (CEB, 1000 m above sea level, Dagestan, Russia ) to search for plants with a high yield of essential oil with a maximum content of valuable biologically active components and expand the growing area of this type of thyme.
Material and methods. The aerial part of the Thymus pulchellus introduced at the CEB was collected in the flowering phase. The essential oil was obtained by hydrodistillation (Clevenger method) for 2 hours from dry raw materials prepared by conventional methods. Component qualitative and quantitative analysis of the isolated essential oil samples was carried out by chromatography-mass spectrometry on a Shimadzu GCMSQP2010plus on a Supelco SLB TM-5 ms column (30 m × 0.25 mm × 0.25 μm) in split mode.
Results. The EF yield was 0.26% in terms of air-dry raw materials. Using chromatography-mass spectrometry, 56 components were isolated from EF samples, of which 97.67% of compounds were identified.
Conclusions. For the first time, data are presented on the study of the composition of the essential oil from the aerial part of the introduced Dagestan sample of Thymus pulcellus. The resulting essential oil consists mainly of the sesquiterpene fraction (84.12%). Compared to other species, the essential oil of the studied pretty thyme showed a completely different set of main components, among which the main ones are limonene, γ-cadinene, α-muurolol, epicubenol, which allows us to assert the isolation of this species. The results obtained allow us to characterize the essential oil of Thymus pulchellus as a source of valuable components, and the plant itself as a promising medicinal raw material.
Full Text
##article.viewOnOriginalSite##About the authors
F. A. Vagabova
Dagestan Federal Research Center of the Russian Academy of Sciences
Author for correspondence.
Email: fazina@mail.ru
Ph.D. (Eng.), Senior Research Scientist, Laboratory of Phytochemistry and Medical Botany, Mountain Botanical Garden
Russian Federation, MakhachkalaA. M. Aliev
Dagestan Federal Research Center of the Russian Academy of Sciences
Email: aslan4848@yahoo.com
Senior Research Scientist, Head of the Laboratory of Phytochemistry and Medical Botany, Mountain Botanical Garden
Russian Federation, MakhachkalaReferences
- Saleem A., Afzal M., Naveed M., et al. HPLC, FTIR and GC-MS analyses of Thymus vulgaris phytochemicals executing in vitro and in vivo biological activities and effects on COX-1, COX-2 and gastric cancer genes computationally. Molecules. 2022; 27(23): 8512; https://doi.org/10.3390/mole-cules27238512.
- Zeljković S.Ć., Maksimović M. Chemical composition and bioactivity of essential oil from Thymus species in Balkan Peninsula. Phytochemistry Reviews. 2015; 14: 335–352.
- Касумов Ф.Ю. Фитоценотическая роль некоторых видов рода Thymus L. в сложении растительного покрова Кавказа. Бюллетень Ботанического сада института ДВО РАН. 2008; 2: 34–7.
- Giweli A.A., Džamić A.M., Soković M.D., et al. Chemical composition, antioxidant and antimicrobial activities of essential oil of Thymus algeriensis wild-growing in Libya. Cent. Eur. J. Biol. 2013; 8(5): 504–11; https://doi.org/10.2478/s11535-013-0150-0.
- Nabet N., Boudries H., Loupassaki S., et al. Chemical composition, antimicrobial and antioxidant activities of Thymus fontanesii Boiss. et Reut. and Origanum glandulosum Desf. essential oils. International Food Research Journal. 2017; 24(6): 2518–25; http://www.ifrj.upm.edu.my.
- Sidiropoulou E., Marugán-Hernández V., Skoufos I., et al. in vitro antioxidant, antimicrobial, anticoccidial, and anti-inflammatory study of essential oils of Oregano, Thyme, and Sage from Epirus, Greece. Life (Basel). 2022; 12(11): 1783; https://doi.org/10.3390/life12111783.
- Varga E., Bardocz A., Belák A., et al. Antimicrobial activity and chemical composition of Thyme essential oils and the polyphenolic content of different Thymus extracts. Farmacia. 2015; 63(3): 357–361.
- Rasooli I., Mirmostafa S.A. Bacterial susceptibility to and chemical composition of essential oils from Thymus kotschyanus and Thymus persicus. J. Agric. Food Chem. 2003; 51(8): 2200–2205; https://doi.org/ 10.1021/jf0261755.
- Tagnaout I., Zerkani H., Hadi N., et al. Chemical composition, antioxidant and antibacterial activities of Thymus broussonetii Boiss and Thymus capitatus (L.) Hoffmann and Link essential oils. Plants. 2022; 11(7): 954;
- https://doi.org /10.3390/plants11070954.
- Tsiftsoglou O.S., Stagiopoulou R., Krigas N., et al. Exploring the ecological preferences and essential oil variability in wild-growing populations of the endangered local Greek endemic Thymus holosericeus (Lamiaceae). Plants. 2023; 12: 348; https://doi.org/10.3390/plants12020348.
- Figuieredo A.C., Barroso J.G., Pedro L.G., et al. Factors affecting secondary metabolite production in plants: volatile components and essential oils. Flavour Fragr. J. 2008; 23: 213–26.
- Elahian F., Garshasbi M., MehriAsiabar Z., et al. Ecotypic variations affected the biological effectiveness of Thymus daenensis Celak essential oil. Evid Based Complement Alternat Med. 2021; 9: 6686558; https://doi.org/10.1155/2021/6686558.
- Baser K.H.C., Demirci B.I., Kirimer N. et al. The essential oils of Thymus migricus and from Turkey. Flavour and Fragrance Journal. 2001; 17: 41–45; https://doi.org/10.1002/ffj.1036.
- Bigdeloo M., Hadian J., Nazeri V. Composition of essential oil compounds from different populations of Thymus caramanicus Jalas. Journal of Applied Research on Medicinal and Aromatic Plants. 2017; 7: 95–98; https://doi.org/10.1016/ j.jarmap.2017.06.005.
- Федотова И. А., Кустова О. К., Шевчук О. М. и др. Хемотипическое разнообразие представителей рода Thymus L. коллекции Донецкого ботанического сада. Бюлле-тень ГНБС. 2023; 147: 96 107; https://doi.org/ 10.25684/0513-1634-2023-147-96-107.
- Boz I., Burzo I., Zamfirache M.-M., et al. Glandular trichomes and essential oil composition of Thymus pannonicus All. (Lamiaceae). Analele Universită Ġii din Oradea, Fascicula Biologie. 2009; 16(2): 36–39.
- Thymus pulchellus C.A. Mey. Плантариум. Растения и лишайники России и сопредельных стран: открытый онлайн атлас и определитель растений. URL: https://www.plantarium.ru/page/view/item/38431.
- URL: https://cicon.ru/thymus-pulchellus.html.
- Волчанская А.В., Фирсов Г.А. Перспективы дальнейшей интродукции древесных растений Красной книги России в Санкт-Петербурге. Hortusbot. 2014; 9; https://doi.org/10.15393/j4.art.2014.2042 (Volchanskaja A.V., Firsov G.A. Perspektivy dal'nejshej introdukcii drevesnyh rastenij Krasnoj knigi Rossii v Sankt-Peterburge. Hortusbot. 2014; 9; https://doi.org/10.15393/j4.art.2014.2042).
- Государственная фармакопея Российской Федерации. М.: Медицина, 2018; 2; с. 2383–2387. URL:
- http://pharmacopoeia.ru/gosudarstvennaya-farmakopeya-xiii-online-gf-13 online (Gosudarstvennaja farmakopeja Rossijskoj Federacii. M.: Medicina, 2018; 2; s. 2383–2387. URL: http://pharmacopoeia.ru/gosudarstvennaya-farmakopeya-xiii-online-gf-13 online).
- Степаненко И. С., Сяткин С. В., Акулина И. В. и др. Антимикробная активность (+)-лимонена и его произ-водного (+)-1,2-оксидалимонена. Вестник Чувашского университета. 2014; 2: 368–373 (Stepanenko I. S., Sjatkin S. V., Akulina I. V. i dr. Antimikrobnaja aktivnost' (+)-limonena i ego proiz-vodnogo (+)-1,2-oksidalimonena. Vestnik Chuvashskogo universiteta. 2014; 2: 368–373).
Supplementary files
