Effect of metabolic type of obesity on blood microbiome
- Authors: Shestopalov A.V1,2,3, Kolesnikova I.M3, Gaponov A.M1,4, Grigoryeva T.V5, Khusnutdinova D.R5, Kamaldinova D.R5, Volkova N.I6, Makarov V.V7, Yudin S.M7, Rumyantsev A.G2,3, Rumyantsev S.A1,3
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Affiliations:
- Center for Molecular Health
- Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology
- N.I. Pirogov Russian National Research Medical University
- V.A. Negovsky Research Institute of General Reanimatology, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology
- Kazan (Volga Region) Federal University
- Rostov State Medical University
- Center for Strategic Planning and Management of Medical and Biological Health Risks
- Issue: Vol 25, No 2 (2022)
- Pages: 35-41
- Section: Articles
- URL: https://ogarev-online.ru/1560-9596/article/view/143477
- DOI: https://doi.org/10.29296/25877313-2022-02-06
- ID: 143477
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Abstract
Background. Recent studies have shown a significant role of the gut microbiome in various pathologies including obesity. It is assumed that the gut microbiome is one of the sources for the formation of the blood microbiome. Obesity is associated with changes in the gut microbiome, which may also affect the blood microbiome. It is customary to distinguish between metabolically healthy obesity (MHO) and metabolically unhealthy obesity (MUHO) depending on the metabolic complications risk. Aim. To study the influence of the metabolic phenotype of obesity on the blood microbiome formation. Materials and methods. The study included 116 healthy donors and 101 obese patients. Depending on the metabolic type of obesity, the obese patients were divided into subgroups with metabolically healthy obesity (MHO, n=36) and metabolically unhealthy obesity (MUHO, n=53). Quantitative and qualitative assessment of the blood microbiome was based on metagenomic analysis. Blood samples were used to isolate DNA and perform sequencing of the variable v3-v4 region of the 16S rRNA gene. Results. In patients with MHO, the characteristics of the alpha-diversity of the blood microbiome were like those of healthy donors. However, MUHO is associated with an increase in the blood microbial diversity. The main phyla of the blood microbiome were Bacteroidetes, Firmicutes, Proteobacteria, and Actinobacteria. Cyanobacteria, TM7, Thermi, Verrucomicrobia, Chloroflexi, Acidobacteria, Planctomycetes, Gemmatimonadetes, and Tenericutes were found to be less significant phyla of the blood microbiome. Phyla Acidobacteria, TM7 and Verrucomicrobia were more often isolated in blood samples of patients with MUHO compared with healthy donors. Conclusion. MUHO linked to increased diversity of the blood microbiome. This appears to be due to increased microbial translocation from the intestine and non-intestinal sources.
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##article.viewOnOriginalSite##About the authors
A. V Shestopalov
Center for Molecular Health; Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology; N.I. Pirogov Russian National Research Medical University
Email: ir.max.kolesnikova@gmail.com
Dr.Sc. (Med.), Professor, Deputy Director, Center of Digital and Translational Biomedicine; Director, Department of Postgraduate Education, Residency, Postgraduate Studies; Head of Department of Biochemistry and Molecular Biology, Medical faculty
Russian Federation, Moscow, RussiaI. M Kolesnikova
N.I. Pirogov Russian National Research Medical University
Email: ir.max.kolesnikova@gmail.com
Senior Lecturer, Department of Biochemistry and Molecular Biology, Medical Faculty
Moscow, RussiaA. M Gaponov
Center for Molecular Health; V.A. Negovsky Research Institute of General Reanimatology, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology
Email: ir.max.kolesnikova@gmail.com
Ph.D. (Med.), Head of Department of Infectious Immunology, Center of Digital and Translational Biomedicine; Leading Researcher
Moscow, RussiaT. V Grigoryeva
Kazan (Volga Region) Federal University
Email: ir.max.kolesnikova@gmail.com
Ph.D. (Biol.), Senior Research Scientist, Director of Interdisciplinary Centre for Proteomic Research, Institute of Fundamental Medicine and Biology
Kazan, RussiaD. R Khusnutdinova
Kazan (Volga Region) Federal University
Email: ir.max.kolesnikova@gmail.com
Junior Research Scientist, Research Laboratory «Omics technologies», Institute of Fundamental Medicine and Biology
Kazan, RussiaD. R Kamaldinova
Kazan (Volga Region) Federal University
Email: ir.max.kolesnikova@gmail.com
Research Scientist
Kazan, RussiaN. I Volkova
Rostov State Medical University
Email: ir.max.kolesnikova@gmail.com
Dr.Sc. (Med.), Professor, Vice-rector for Scientific Work, Head of Department of Internal Diseases №3
Rostov-on-Don, RussiaV. V Makarov
Center for Strategic Planning and Management of Medical and Biological Health Risks
Email: ir.max.kolesnikova@gmail.com
Ph.D. (Biol.), Head of Department of Analysis and Forecasting of Medical and Biological Risks to Human Health
Moscow, RussiaS. M Yudin
Center for Strategic Planning and Management of Medical and Biological Health Risks
Email: ir.max.kolesnikova@gmail.com
Dr.Sc. (Med.), Professor, General Director
Moscow, RussiaA. G Rumyantsev
Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology; N.I. Pirogov Russian National Research Medical University
Email: ir.max.kolesnikova@gmail.com
Dr.Sc. (Med.), Professor; Honorary Professor, Department of Oncology, Hematology and Radiotherapy, Pediatric Faculty
Moscow, RussiaS. A Rumyantsev
Center for Molecular Health; N.I. Pirogov Russian National Research Medical University
Author for correspondence.
Email: ir.max.kolesnikova@gmail.com
Dr.Sc. (Med.), Professor, Corresponding Member of the Russian Academy of Sciences, Director; Head of Department of Oncology, Hematology and Radiotherapy, Pediatric Faculty
Moscow, RussiaReferences
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