Radioimmunochemical studies for development of a solid-phase radioimmunoassay system using coated beads for the assessment of human chorionic gonadotropin

Resumo

A method was developed for immobilization of prepared human chorionic gonadotropin (β-hCG) antisera on fresh plastic beads through a covalent immune bridge. Such covalent immune-coated antisera on plastic beads were used in the development of a noncentrifugation radioimmunoassay. ¹²⁵I-β-hCG was prepared using different oxidizing agents. The solid-phase RIA system developed was found to well compete with the conventional method.