Changes in the Composition and Fluorescent Properties of Bisretinoids in the Retina and the Retinal Pigment Epithelium of the Mouse Eye under Exposure to Ionizing Radiation

Abstract

Fluorescence and chromatographic analysis of bisretinoids from the retina and retinal pigment epithelium of mouse eyes was carried out before and after exposure to accelerated protons in the Bragg peak. It has been shown that ionizing radiation at doses of 1–4 Gy leads to a shift in the short-wave region of the maximum of the fluorescence spectrum in the chloroform extract obtained from both the retinal pigment epithelium and the retina. Chromatographic analysis of these extracts has shown a change in the relative content of individual bisretinoids. The obtained spectral and chromatographic data indicate that the exposure of mice to accelerated protons in the Bragg peak at doses of 1–4 Gy leads to radiation oxidation of bisretinoids in eye tissues.