CHANGES IN PHAGOCYTOSIS PARAMETERS WITH A MARKER OF PHAGOSOME ACIDIFICATION pHrodo™ UNDER-ZYMOSAN-INDUCED PERITONITIS IN RATS
- Authors: Shilov S.J.1,2, Shilov J.I.1,2, Tulyaev Y.A.1, Barkov S.Y.2
-
Affiliations:
- “Institute of Ecology and Genetics of Microorganisms of the Ural Branch of the Russian Academy of Sciences” – a branch of the PFRC UB RAS
- Acad. E. A. Wagner Perm State Medical University
- Issue: Vol 22, No 2-2 (2019)
- Pages: 984-986
- Section: ORIGINAL ARTICLES
- URL: https://ogarev-online.ru/1028-7221/article/view/120169
- DOI: https://doi.org/10.31857/S102872210006497-1
- ID: 120169
Cite item
Full Text
Abstract
The changes in phagocytosis under 12-hour zymosan-induced peritonitis in rats were investigated using flow laser cytometry using zymosan labeled with pH-sensitive flurochrome green pHrodo™ (Thermo Fisher Scientifi c). An increase in the absolute parameters of phagocytosis was found in the study of peritoneal exudate cells, blood leukocytes and bone marrow cells in rats with peritonitis in comparison with intact animals.
About the authors
S. Ju. Shilov
“Institute of Ecology and Genetics of Microorganisms of the Ural Branch of the Russian Academy of Sciences” – a branch of the PFRC UB RAS;Acad. E. A. Wagner Perm State Medical University
Author for correspondence.
Email: stshilov@mail.ru
PhD, MD, Junior Researcher,
Perm
Russian FederationJu. I. Shilov
“Institute of Ecology and Genetics of Microorganisms of the Ural Branch of the Russian Academy of Sciences” – a branch of the PFRC UB RAS;Acad. E. A. Wagner Perm State Medical University
PhD, MD, Associate Professor, Leading Researcher,
Perm
Russian FederationYa. A. Tulyaev
“Institute of Ecology and Genetics of Microorganisms of the Ural Branch of the Russian Academy of Sciences” – a branch of the PFRC UB RAS
Aspirant,
Perm
Russian FederationS. Yu. Barkov
Acad. E. A. Wagner Perm State Medical University
Student,
Perm
Russian FederationReferences
- Zhang R., Kobayashi Y., Kazumura K., Tsuchiya H., Morishita N., Inagawa H., Soma G. Development of an evaluation device for phagocytic activity of new phagocytes using simple and pH-sensitive particles that do not require pre-treatment. Anticancer Res. 2016, 36(7), 3613–3618.
- Kapellos T. S., Taylor L., Lee H., Cowley S. A., James W. S.., Iqbal A. J., Greaves D. R. A novel real time imaging platform to quantify macrophage phagocytosis. Biochemical Pharmacology 2016, 116, 107–119.
- Шилов Ю. И., Шилов С. Ю., Жукова А. Е., Барков С. Ю., Петухова А. А. Изменение уровня ацидификации фагосом у крыс в зависимости от фазы эстрального цикла. Российский иммунологический журнал 2016, том 10 (19), № 2 (1), 183–184.
Supplementary files
