CYTOKINE PROFILE OF MICE IMMUNIZED BY COMPLEX OF RECOMBINANT OPRF AND ATOX ANTIGENS OF PSEUDOMONAS AERUGINOSA

Currently, infections caused by Pseudomonas aeruginosa are an important medical problem. Their treatment is extremely difficult because of the broad antibiotic resistance of the pathogen. Objective: to study the effect of immunization with a recombinant antigens of Pseudomonas aeruginosa on the concentration of cytokines in the serum of mice. Materials and methods. Mice were injected intraperitoneally with recombinant 25 μg OprF and 50 μg of toxoid. The cytokine responses of mice were monitored by using flow cytometry analysis at 2, 4, 6 and 24 hours after immunization («FlowCytomix Mouse Th1/Th2 10 plex»). Results. The dynamics of the cytokine levels in the sera of mice was revealed: after 4 hours IL-1α, IL-5; 8 hours later, Π,-1β, IL-2, IL-4, and Th17/Th21/Th22 cytokines-IL-17A and IL-22 and IL-21; 24 hours later, IL-6, IL-10, IL-12p70, TNF-a and IFN-γ. Conclusion. Candidate vaccine against Pseudomo-nas aeruginosa based on the recombinant proteins OprF and aTox activates both cellular and humoral immune system with induction of Th1, Th2 and Th17 responses.

синегнойная палочка, рекомбинантные белки, цитокины, Pseudomonas aeruginosa, recombinant proteins, cytokines