EXPRESSION OF VASCULAR ENDOTHELIAL GROWTH FACTOR RECEPTORS 1 (VEGFR-1) AND THEIR ROLE IN THE REGULATION OF T CELL PROLIFERATION
- Authors: Tashkina E.A.1, Leplina O.Y.1, Batorov E.V.1, Ostanin A.A.1, Pasman N.M.2
-
Affiliations:
- Federal Budget Institution of Science «Research Institute of Fundamental and Clinical Immunology»
- Novosibirsk National Research State University
- Issue: Vol 22, No 2-2 (2019)
- Pages: 942-944
- Section: ORIGINAL ARTICLES
- URL: https://ogarev-online.ru/1028-7221/article/view/119982
- DOI: https://doi.org/10.31857/S102872210006534-2
- ID: 119982
Cite item
Full Text
Abstract
The expression of vascular endothelial growth factor receptors-1 (VEGFR-1) on CD4+ and CD8+ donor T lymphocytes in mononuclear cell (MNC) cultures, as well as the eff ect of placental growth factor (PlGF) on T cell proliferation have been investigated. Freshly isolated T cells had low expression of VEGFR-1, which increased during spontaneous cultivation of T cells and most strongly upon activation of T lymphocytes with aCD3 antibodies. The maximum increase in VEGFR-1 – positive CD4+ and CD8+ T cells is observed at 48 hours. PlGF in a wide dose range inhibits the proliferation of aCD3- stimulated T cells, suppressing CD4+ and CD8+ T lymphocyte division to the same extent.
Keywords
About the authors
E. A. Tashkina
Federal Budget Institution of Science «Research Institute of Fundamental and Clinical Immunology»
Author for correspondence.
Email: ct_lab@mail.ru
Postgraduate Student, Laboratory of Cellular Immunotherapy,
Novosibirsk
Russian FederationO. Yu. Leplina
Federal Budget Institution of Science «Research Institute of Fundamental and Clinical Immunology»
Email: fake@neicon.ru
PhD, MD (Medicine), Leading Research Associate, Laboratory of Cellular Immunotherapy,
Novosibirsk
Russian FederationE. V. Batorov
Federal Budget Institution of Science «Research Institute of Fundamental and Clinical Immunology»
Email: fake@neicon.ru
PhD (Medicine), Research Associate, Laboratory of Cellular Immunotherapy,
Novosibirsk
Russian FederationA. A. Ostanin
Federal Budget Institution of Science «Research Institute of Fundamental and Clinical Immunology»
Email: fake@neicon.ru
PhD, MD, Professor (Medicine), Main Research Associate, Laboratory of Cellular Immunotherapy,
Novosibirsk
Russian FederationN. M. Pasman
Novosibirsk National Research State University
Email: fake@neicon.ru
PhD, MD, Professor (Medicine), Head of Department of Obstetrics and Gynecology,
Novosibirsk
Russian FederationReferences
- Ozao-Choy J., Ma G., Kao J., Wang G., Meseck M., Sung M., Schwartz M., Divino C., Pan P., Chen S. The novel role of tyrosine kinase inhibitor in the reversal of immune suppression and modulation of tumor microenvironment for immune-based cancer therapies. Cancer Res. 2009, 69(6), 2514–2210.
- Voron T., Marcheteau E., Pernot S., Colussi O., Tartour E., Taieb J., Terme M. Control of the immune response by pro-angiogenic factors. Front Oncol. 2014, 4, 70.
- Lin Y-L., Liang Y-C., Chiang B-L. Placental growth factor down-regulates type 1 T helper immune response by modulating the function of dendritic cells. Journal of Leukocyte Biology, 2007, 82, 1473–2480.
- Ziogas A., Gavalas N., Tsiatas M., Tsitsilonis O., Politi E., Terpos E., Rodolakis A., Vlahos G., Thomakos N., Haidopoulos D., Antsaklis A., Dimopoulos M., Bamias A. VEGF directly suppresses activation of T cells from ovarian cancer patients and healthy individuals via VEGF receptor Type 2. Int J Cancer. 2012,. 130; 4, 857–864.
- Basu A., Hoerning A., Datta D., Edelbauer M., Stack M., Calzadilla K., Pal S., Briscoe D. Cutting edge: vascular endothelial growth factor-mediated signaling in human CD45RO+CD4+ T cells promotes Akt and ERK activation and costimulates IFN-gamma production. J Immunol. 2010, 184; 2, 545–549.
Supplementary files
