Evaluation of Expression of a New Isoform DjGDNF47 in Neural Cells

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Resumo

Glial cell line- derived neurotrophic factor (GDNF) is a protein belonging to a large group of proteins called neurotrophic factors. GDNF ensures the development of the nervous system during embryogenesis, supports the process of neuronal differentiation in the adult organism, and promotes the survival of neurons throughout their lifetime. Early experiments as well as clinical trials showed the possibility of using GDNF as a therapeutic factor for adjuvant therapy of a number of diseases of the nervous system: Parkinson's disease, ischaemic stroke, spinal cord disorders. Failures in clinical trials have led to a search for possible alternatives that would allow GDNF to be used effectively in therapy. One such option was the search for GDNF isoforms and the study of their possible neuroprotective and neuroinducer potential. A new splice variant of GDNF was discovered and named djGDNF47. In this work, we show the localisation of a novel GDNF isoform, djGDNF47, in the normal brain of healthy rats 10 days old from birth, as well as in adult individuals. We show that djGDNF47 is predominantly expressed in young neurons of the subventricular zone (SVZ). The expression of djGDNF47 was also evaluated in pathology cells (human glioblastoma cell culture), where its expression is detected by CD133+ conditional stemness cells.

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Sobre autores

V. Parshina

Institute of Higher Nervous Activity and Neurophysiology Russian Academy of Sciences

Email: parshina-v-v@yandex.ru
Moscow, Russia

D. Shamadykova

Institute of Higher Nervous Activity and Neurophysiology Russian Academy of Sciences

Moscow, Russia

V. Kolesnikova

Institute of Higher Nervous Activity and Neurophysiology Russian Academy of Sciences

Moscow, Russia

L. Fab

Institute of Higher Nervous Activity and Neurophysiology Russian Academy of Sciences

Moscow, Russia

A. Revishchin

Institute of Higher Nervous Activity and Neurophysiology Russian Academy of Sciences

Moscow, Russia

G. Pavlova

Institute of Higher Nervous Activity and Neurophysiology Russian Academy of Sciences; N.N. Burdenko National Medical Research Center of Neurosurgery" of the Ministry of Health of the Russian Federation

Moscow, Russia

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