The Effect of Repeated Argon Inhalations on the Behavior of Wistar Rats in a Neonatal Hypoxia Model

Cover Page

Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription Access

Abstract

The inert gas argon has recently attracted attention from researchers due to its pronounced neuroprotective properties. These properties are mediated by argon's ability to suppress inflammatory processes and apoptosis. However, the therapeutic potential of argon application during the neonatal developmental period remains poorly studied. The early neonatal period is characterized by heightened sensitivity of the central nervous system to damaging factors, particularly neonatal hypoxia. This study evaluated the ability of repeated argon inhalations to interact with the effects of neonatal hypoxia at the behavioral level and exert delayed effects on caspase-3 expression. Inhalations were administered using an argon-enriched gas mixture (74% Ar, 21% O2, 5% N2) or artificial air from postnatal days (PND) 3 to 10 in a model of single normobaric hypoxia (8% O2 for 2 hours on PND 2). The results showed that argon inhalations marginally increased the number of orienting head turns in rat pups on PND 5 but significantly reduced the speed of righting reflex execution in the negative geotaxis test on PND 11. Repeated argon inhalations led to decreased exploratory activity in the Open Field test (PND 27) and impaired skill acquisition in the Complex Food Maze. Perinatal hypoxia, in turn, reduced the quality of vestibulospinal reflex execution and locomotion in the early postnatal period. In the late postnatal period, hypoxia suppressed exploratory activity in the Elevated Plus Maze and worsened learning ability in the Complex Food Maze. Thus, argon application during this developmental phase may exert undesirable effects on the emotional status of the animals. No differences in hippocampal caspase-3 expression levels on PND 55 were observed between the groups.

About the authors

I. A. Kabiolskiy

Lomonosov Moscow State University; LLC “InertGas Medical”

Email: ilyakab1999@gmail.com
Moscow, Russia

A. S. Degtyar

Lomonosov Moscow State University

Moscow, Russia

P. D. Panasenkov

LLC “InertGas Medical”

Moscow, Russia

A. V. Loboda

LLC “InertGas Medical”

Moscow, Russia

S. V. Potapov

LLC “InertGas Medical”

Moscow, Russia

N. Yu. Sarycheva

Lomonosov Moscow State University

Moscow, Russia

V. A. Dubynin

Lomonosov Moscow State University

Moscow, Russia

References

  1. Kabiolskiy I.A., Simonenko S.D., Sarycheva N.U., Dubynin V.A. // J. Evol. Biochem. Phys. 2024. V. 110. № 10. P. 1582–1601.
  2. Jawad N., Rizvi M., Gu J., Adeyi O., Tao G., Maze M., Ma D. // Neurosci. Lett. 2009. V. 460. № 3. P. 232–236.
  3. Ma S., Chu D., Li L., Creed J.A., Ryang Y.M., Sheng H., Yang W., Warner D.S., Turner D.A., Hoffmann U. // Crit. Care. Med. 2019. V. 47. № 8. P. 693–699.
  4. Behnke A.R., Yarbrough O.D. // Am. J. Physiol. 1939. V. 126. № 2. P. 409–415.
  5. Ulbrich F., Kaufmann K., Roesslein M., Wellner F., Auwärter, V., Kempf, J., Loop T., Buerkle H., Goebel U. // PLoS One. 2015. V. 10. № 12. P. e0143887.
  6. Ulbrich F., Schallner N., Coburn M., Loop T., Lagrèze W.A., Biermann J., Goebel U. // PLoS One. 2014. V. 9. № 12. P. e115984.
  7. David H.N., Haelewyn B., Degoulet M., Colomb D.G.Jr., Risso J.J., Abraini J.H. // PLoS One. 2012. V. 7 № 2. P. e30934.
  8. Nowrangj D.S., Tang J., Zhang J.H. // Med. Gas. Res. 2014. V. 4. № 3.
  9. Scheid S., Lejarre A., Wollborn J., Buerkle H., Goebel U., Ulbrich F. // Neural. Regen. Res. 2023. V. 18. № 6. P. 1371–1377.
  10. Zhang W., Xiao D., Mao Q., Xia H. // Signal. Transduct. Target. Ther. 2023. V. 8. № 1. P. 267.
  11. Usui N., Kobayashi H., Shimada S. // Int. J. Mol. Sci. 2023. V. 24. № 6. P. 5487.
  12. Vázquez-González D., Carreón-Trujillo S., Alvarez-Arellano L., Abarca-Merlin D. M., Domínguez-López P., Salazar-García M., Corona J.C. // Adv. Exp. Med. Biol. 2023. V. 1411. P. 327–356.
  13. Eltzschig H.K., Carmeliet P. // N. Engl. J. Med. 2011. V. 364. № 7. P. 656–665.
  14. Hu F., Mu R., Zhu J., Shi L., Li Y., Liu X., Shao W., Li G., Li M., Su Y., Cohen P.L., Qiu X., Li Z. // Ann. Rheum. Dis. 2014. V. 73. № 5. P. 928–936.
  15. Gedzun V.R., Khukhareva D.D., Sarycheva N.Y., Kotova M.M., Kabiolsky I.A., Dubynin V.A. // Neurosci. Behav. Physiol. 2022. V. 72. № 4. P. 457–470.
  16. Граф А.В., Гончаренко Е.Н., Соколова Н.А., Ашмарин И.П. // Нейрохимия. 2008. Т. 25. № 1. С. 11–16.
  17. Суханова Ю.А., Себенцова Е.А., Левицкая Н.Г. // Нейрохимия. 2016. Т. 33. № 4. С. 276–292.
  18. van de Looij Y., Chatagner A., Hüppi P.S., Gruetter R., Sizonenko S.V. // Magn. Reson. Med. 2011. V. 65. № 2. P. 305–312.
  19. Yao L., Kan E.M., Lu J., Hao A., Dheen S.T., Kaur C., Ling E.A. // J. Neuroinflammation. 2013. V. 10. № 1. P. 785.
  20. Balsamo F., Li B., Chuslip S., Lee D., Biouss G., Lee C., Maynes J.T., Pierro A. // Pediatr. Surg. Int. 2023. V. 40. № 1. P. 21.
  21. Zhao H., Mitchell S., Koumpa S., Cui Y.T., Lian Q., Hagberg H., Johnson M.R., Takata M., Ma D. // Anesthesiology. 2016. V. 125. № 1. P. 180–192.
  22. Устинович Ю.А., Жевнеронок И.В., Кизатова С.Т. // Репродуктивное Здоровье. Восточная Европа. 2024. Т. 14. № 2. С. 243–263
  23. Sukhanova I.A., Sebentsova E.A., Khukhareva D.D., Manchenko D.M., Glazova N.Y., Vishnyakova P.A., Inozemtseva L.S., Dolotov O.V., Vysokikh M.Y., Levitskaya N.G. // Behav. Brain. Res. 2018. V. 17. № 350. P. 87–98.
  24. Altman J., Sudarshan K. // Anim. Behav. 1975. V. 23. P. 896–920.
  25. Paxinos G., Watson C. // New York: Academic Press, 2009. 340 P.
  26. Sun J., Xie C., Liu W., Lu D., Qiao W., Huang Q., Huo Z., Shen H., Lin Z. // Int. J. Mol. Med. 2012. V. 30. № 4. P. 739–46.
  27. Li J., Han B., Ma X., Qi S. // Brain. Res. 2010. V. 14. № 1356. P. 11–23.
  28. Koehn L.M., Nguyen K., Chen X., Santoso A., Tucker R., Lim Y.P., Stonestreet B.S. // Int. J. Mol. Sci. 2022. V. 23. № 21. P. 13473.
  29. Balakina M.E., Degtyareva E.V., Nekrasov M.C., Brus T.V., Purveev S.S. // Russ. Biomed. Res. 2021. V. 6. №2. P. 27–37. (in Rus)
  30. Erdi–Krausz G., Rocha R., Brown A., Myneni A., Lennartsson F., Romsauerova A., Cianfaglione R., Edmonds C.J., Vollmer B. // Eur. J. Paediatr. Neurol. 2021. – V. 35. – P. 74–81.
  31. Edmonds C.J., Helps S.K., Hart D., Zatorska A., Gupta N., Cianfaglione R., Vollmer B. // Eur. J. Paediatr. Neurol. 2020. V. 27. P. 78–85.
  32. Хухарева Д.Д., Суханова Ю.А., Себенцова Е.А., Левицкая Н.Г. // Рос. физиол. журн. им. И.М. Сеченова. 2021. Т. 107. № 2. С. 187–202.
  33. Miguel P.M., Schuch C.P., Rojas J.J., Carletti J.V., Deckmann I., Martinato L.H.M., Pires A.V., Bizarro L., Pereira L.O. // Behav. Neurosci. 2015. V. 129. № 3. P. 309–320.
  34. Sanches E.F., Van de Looij Y., Toulotte A., Sizonenko S.V., Lei H. // Front. Physiol. 2019. V. 10. P. 634.
  35. Wu Y., Wei H., Li P., Zhao H., Li R., Yang F. // Front. Pediatr. 2022. V. 10. P. 791815.
  36. Álvarez-García M., Cuellar-Flores I., Sierra-García P., Martínez-Orgado J. // PLoS One. 2022. V. 17. № 1. P. e0263055.
  37. Johnston A.L., File S.E. // Physiol. Behav. 1991. V. 49. № 2. P.245–250.
  38. Sanches E.F., Arteni N., Nicola F., Aristimunha D., Netto C.A. // Neuroscience. 2015. V. 290. P. 581–593.
  39. Schreglmann M., Ground A., Vollmer B., Johnson M.J. // Acta. Paediatr. 2020. V. 109. № 1. P. 20–30
  40. Machado D.N., Durán–Carabali L.E., Odorcyk F.K., Carvalho A.V.S., Martini A.P.R., Schlemmer L.M., de Mattos M., Bernd G.P., Dalmaz C., Netto C.A. // Neurotox. Res. 2023. V. 41. № 6. P. 526–545.
  41. Cheng J., Zheng B., Luo S., Yuan Y., Wu X., Jiang Z., Li X. // J. Mol. Cell. Biol. 2025. P. mjaf006.
  42. Zhuang L., Yang T., Zhao H., Fidalgo A.R., Vizcaychipi M.P., Sanders R.D., Yu B., Takata M., Johnson M.R., Ma D. // Crit. Care. Med. 2012. V. 40. № 6. P. 1724-1730.
  43. Loetscher P.D., Rossaint J., Rossaint R., Weis J., Fries M., Fahlenkamp A., Ryang Y.M., Grottke O., Coburn M. // Crit. Care. 2009. V. 13. № 6. P. 206.
  44. Kudryashov I.E., Onufriev M.V., Kudryashova I.V., Gulyaeva N.V. // Brain. Res. Dev. Brain. Res. 2001. V. 132. № 2. P. 113-120.
  45. Kudryashova I.V., Stepanichev M.Y., Gulyaeva N.V. // Neurosci. Behav. Physiol. 2009. V. 39. № 1. P. 65-72.
  46. Dubrovskaya N.M., Vasilev D.S., Tikhonravov D.L., Tumanova N.L., Zhuravin I.A. // Neurosci. Behav. Physiol. 2019. V. 49. P. 514-521.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2025 Russian Academy of Sciences

Согласие на обработку персональных данных

 

Используя сайт https://journals.rcsi.science, я (далее – «Пользователь» или «Субъект персональных данных») даю согласие на обработку персональных данных на этом сайте (текст Согласия) и на обработку персональных данных с помощью сервиса «Яндекс.Метрика» (текст Согласия).