Characteristics of  Bacillus cereus  complex Group Strains Isolated from Permafrost in Yakutia for Assessment of Microbiological Risks During Climate Change

Y. O. Goncharova; Гончарова Ю. О.; V. S. Timofeev; Тимофеев В. С.; A. V. Brushkov; Брушков А. В.; T. B. Kravchenko; Кравченко Т. Б.; I. V. Bahtejeva; Бахтеева И. В.; G. M. Titareva; Титарева Г. М.; V. I. Solomentsev; Соломенцев В. И.; A. A. Sizova; Сизова А. А.; A. G. Bogun; Богун А. Г.; K. V. Khlopova; Хлопова К. В.; R. I. Mironova; Миронова Р. И.; V. V. Evseeva; Евсеева В. В.; S. G. Ignatov; Игнатов С. Г.

doi:10.31857/S0555109923060053

Characteristics of Bacillus cereus complex Group Strains Isolated from Permafrost in Yakutia for Assessment of Microbiological Risks During Climate Change

Authors: Goncharova Y.O.¹, Timofeev V.S.¹, Brushkov A.V.²^,3, Kravchenko T.B.¹, Bahtejeva I.V.¹, Titareva G.M.¹, Solomentsev V.I.¹, Sizova A.A.¹, Bogun A.G.¹, Khlopova K.V.¹, Mironova R.I.¹, Evseeva V.V.¹, Ignatov S.G.¹^,2
Affiliations:
1. State Research Center for Applied Microbiology and Biotechnology
2. Moscow State University M.V. Lomonosov
3. Tyumen State University
Issue: Vol 59, No 6 (2023)
Pages: 589-598
Section: Articles
URL: https://ogarev-online.ru/0555-1099/article/view/232494
DOI: https://doi.org/10.31857/S0555109923060053
EDN: https://elibrary.ru/CVASHJ
ID: 232494

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Abstract
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Abstract

Strains of Bacillus genus were isolated from soil samples in the permafrost region (Yakutia, Russia). The phenotypic characteristics of the strains are given. The analysis of the obtained data made it possible to assign them to the group Bacillus cereus complex. PCR analysis made it possible to determine the profile of B. cereus toxin synthesis genes in the genomes of the studied strains. Genetic characterization was obtained by RAPD genotyping and using MLVA loci used for genotyping of the anthrax pathogen. The results of genotyping at different levels of resolution made it possible to differentiate the studied strains from the B. anthracis species, to show their intraspecific genetic differences and the degree of relationship. Whole genome sequencing was carried out, based on the data of which MLST genotyping was carried out, which revealed 2 known sequence types and one new one, described for the first time in this work. The results obtained are of practical importance and are extremely interesting from the point of view of the evolution and phylogeography of the B. cereus complex group, since the fact that strains were isolated from permafrost suggests that their age may be much older than expected.

Keywords

Bacillus cereus complex, Bacillus anthracis, toxins, genotyping, MLVA, MLST, RAPD, permafrost

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