Ectopic Expression of Human, Mouse, and Fruit Fly Aryl Hydrocarbon Receptor in  Drosophila   melanogaster  Tissues

D. Yu. Baranova; Баранова Д. Ю.; A. A. Akishina; Акишина А. А.; R. O. Cherezov; Черезов Р. О.; D. A. Kulikova; Куликова Д. А.; I. B. Mertsalov; Мерцалов И. Б.; O. B. Simonova; Симонова О. Б.; J. E. Vorontsova; Воронцова Ю. Е.

doi:10.31857/S0475145023060034

Ectopic Expression of Human, Mouse, and Fruit Fly Aryl Hydrocarbon Receptor in Drosophila melanogaster Tissues

Authors: Baranova D.Y.¹, Akishina A.A.², Cherezov R.O.², Kulikova D.A.², Mertsalov I.B.², Simonova O.B.², Vorontsova J.E.²
Affiliations:
1. Institute of Biology and Chemistry Moscow Pedagogical State University
2. Koltzov Institute of Developmental Biology of Russian Academy of Sciences
Issue: Vol 54, No 6 (2023)
Pages: 377-383
Section: Original study articles
URL: https://ogarev-online.ru/0475-1450/article/view/232033
DOI: https://doi.org/10.31857/S0475145023060034
EDN: https://elibrary.ru/GEJATJ
ID: 232033

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Abstract

The aryl hydrocarbon receptor (AHR) is a ligand-dependent transcription factor and its target genes play a fundamental role in detoxification, regulation of developmental processes, maintenance of homeostasis, as well as in the occurrence of oncological and autoimmune diseases and drug metabolism. The high conservatism of vertebrate AHR allowed us to study its functions in vivo using transformed Drosophila melanogaster fruit flies with human or mouse AHR gene and compare the ectopic effect of their expression with the expression of spineless gene, Drosophila AHR homologue. This work demonstrates for the first time that vertebrate AHR exhibits its functional activity in Drosophila embryogenesis, in leg imaginal discs and in somatic cells of female reproductive system in the absence of exogenous ligands.

Keywords

aryl hydrocarbon receptor AHR, spineless, ectopic expression, ligand, Drosophila melanogaster

References

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