МИКРОБНЫЕ 2-ЕНОАТРЕДУКТАЗЫ, СОДЕРЖАЩИЕ КОВАЛЕНТНО СВЯЗАННЫЙ ФЛАВИНМОНОНУКЛЕОТИД

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Аннотация

Флавины FMN и FAD являются простетическими группами многих ферментов и могут присоединяться к белкам как ковалентно, так и нековалентно. Ковалентное присоединение FMN к остаткам Thr или Ser через фосфатную группу катализируется недавно открытым ферментом флавинтрансферазой. Среди ферментов, содержащих фосфоэфирно связанный FMN, наиболее широко представлены разнообразные микробные 2-еноатредуктазы, катализирующие восстановление непредельных карбоновых кислот, таких как фумаровая, акриловая, коричная, урокановая и другие. Настоящий обзор посвящён этим ферментам и в нём рассмотрены их типы по доменной организации и внутриклеточной локализации, структурные основы субстратной специфичности, каталитический механизм, роль, а также значение и эволюционное происхождение ковалентного присоединения FMN как простетической группы.

Об авторах

А. В Богачев

Московский государственный университет им. М.В. Ломоносова; НИИ физико-химической биологии им. А.Н. Белозерского

Email: bogachev@belozersky.msu.ru
Москва, Россия

А. А Байков

Московский государственный университет им. М.В. Ломоносова; НИИ физико-химической биологии им. А.Н. Белозерского

Москва, Россия

В. А Анашкин

Московский государственный университет им. М.В. Ломоносова; НИИ физико-химической биологии им. А.Н. Белозерского

Москва, Россия

Ю. В Берцова

Московский государственный университет им. М.В. Ломоносова; НИИ физико-химической биологии им. А.Н. Белозерского

Москва, Россия

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