Quantitative and qualitative determination of human papillomavirus DNA in women with cervical lesions
- Авторлар: Bayramova G.R.1, Andreev A.O.1, Ilyasova N.A.1, Tregubova A.V.1, Badlaeva A.S.1, Trofimov D.Y.1
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Мекемелер:
- Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
- Шығарылым: № 1 (2024)
- Беттер: 102-109
- Бөлім: Original Articles
- URL: https://ogarev-online.ru/0300-9092/article/view/275236
- DOI: https://doi.org/10.18565/aig.2023.260
- ID: 275236
Дәйексөз келтіру
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Аннотация
Objective: This study aimed to investigate the relationship between quantified human papillomavirus (HPV) viral load and the severity of cervical lesions.
Materials and methods: Data from 819 HPV-positive women aged 18–81 years were analyzed. Patients were categorized into three groups based on histologically verified diagnoses. Group 1 comprised 186 patients with chronic cervicitis, Group 2 included 341 patients with low-grade squamous intraepithelial lesions (LSIL), and Group 3 included 292 patients with high-grade squamous intraepithelial lesions (HSIL). HPV DNA testing was conducted by real-time PCR to determine the number of genomic equivalents of the virus. The HPV Kvant-21 reagent kit, designed for the detection, typing, and quantification of human papillomavirus DNA with low carcinogenic risk (HPV types 6, 11, 44) and high carcinogenic risk (HPV types 16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, 82), was used for analysis.
Results: The study found that detection of a high viral load was associated with a statistically significantly higher risk of HSIL than LSIL, regardless of HPV genotype (OR=5.26; 95% CI 3.19–8.64). A similar trend was observed with moderate viral loads (OR=2.32; 95% CI 1.78–3.03). Furthermore, LSIL was significantly more common than HSIL when a low viral load was detected (OR=3.09; 95% CI 1.91–4.99). Additionally, a statistically significant positive relationship between the viral load and the degree of cervical involvement was identified for HPV genotypes 16, 18, 31, 39, and 44.
Conclusion: This study highlights the significance of HPV load as an important diagnostic marker. This suggests that the value of HPV load is currently underrated owing to challenges in interpreting the results of quantitative HPV DNA determination and the complexity of conducting research in this domain.
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##article.viewOnOriginalSite##Авторлар туралы
Guldana Bayramova
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Хат алмасуға жауапты Автор.
Email: bayramova@mail.ru
ORCID iD: 0000-0003-4826-661X
Dr. Med. Sci., Merited Doctor of the Russian Federation, Professor at the Department of Obstetrics and Gynecology of the Department of Professional Education, Clinical Care Supervisor at the Research and Outpatient Department
Ресей, 117997, Moscow, Oparina str., 4Alexander Andreev
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: sasha.grash2010@yandex.ru
ORCID iD: 0000-0002-9835-440X
Ph.D. Student, specialty “obstetrics and gynecology”
Ресей, 117997, Moscow, Oparina str., 4Natalya Ilyasova
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: bayramova@mail.ru
ORCID iD: 0000-0003-0665-3515
Researcher at the Department of International Cooperation, Obstetrician-Gynecologist at the Research and Outpatient Department
Ресей, 117997, Moscow, Oparina str., 4Anna Tregubova
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: a_tregubova@oparina4.ru
ORCID iD: 0000-0003-4601-1330
Junior Researcher at the 1st Pathological Department
Ресей, 117997, Moscow, Oparina str., 4Alina Badlaeva
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: bayramova@mail.ru
ORCID iD: 0000-0001-5223-9767
Junior Researcher at the 1st Pathological Department
Ресей, 117997, Moscow, Oparina str., 4Dmitry Trofimov
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: d_trofimov@oparina4.ru
Corresponding Member of the RAS, Professor, Dr. Bio. Sci., Director of the Institute of Reproductive Genetics
Ресей, 117997, Moscow, Oparina str., 4Әдебиет тізімі
- Shen T.-T., Long C.-Y., Wu M.-P. Favorable cervical cancer mortality-to-incidence ratios of countries with good human development index rankings and high health expenditures. BMC Women’s Health. 2023;23(1):284. https://dx.doi.org/10.1186/s12905-023-02423-y.
- Siegel R.L., Miller K.D., Fuchs H.E., Jemal A. Cancer statistics, 2022. CA Cancer J. Clin. 2022;72(1):7-33. https://dx.doi.org/10.3322/caac.21708.
- Sung H., Ferlay J., Siegel R.L., Laversanne M., Soerjomataram I., Jemal A., Bray F. Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J. Clin. 2021;71(3):209-49. https://dx.doi.org/10.3322/caac.21660.
- WHO guideline for screening and treatment of cervical pre-cancer lesions for cervical cancer prevention, second edition. World Health Organization; 2021.
- Portnoy A., Pedersen K., Trogstad L., Hansen B.T., Feiring B., Laake I. et al. Impact and cost-effectiveness of strategies to accelerate cervical cancer elimination: a model-based analysis. Prev. Med. 2021;144:106276. https://dx.doi.org/10.1016/j.ypmed.2020.106276.
- Hall M.T., Simms K.T., Lew J.B., Smith M.A., Brotherton J., Saville M. et al. The projected timeframe until cervical cancer elimination in Australia: a modelling study. Lancet Public Health. 2019;4:19-27. https://dx.doi.org/10.1016/ S2468-2667(18)30183-X.
- Burger E.A., Smith M.A., Killen J., Sy S., Simms K.T., Canfell K., Kim J.J. Projected time to elimination of cervical cancer in the USA: a comparative modelling study. Lancet Public Health. 2020;5(4):e213-e222. https://dx.doi.org/10.1016/S2468-2667(20)30006-2.
- Castanon A., Rebolj M., Sasieni P. Is a delay in the introduction of human papillomavirus-based cervical screening affordable? J. Med. Screen. 2019;26(1):44-9. https://dx.doi.org/10.1177/0969141318800355.
- Kojalo U., Tisler A., Parna K., Kivite-Urtane A., Zodzika J., Stankunas M. et al. An overview of cervical cancer epidemiology and prevention in the Baltic States. BMC Public Health. 2023;23(1):660. https://dx.doi.org/10.1186/ s12889-023-15524-y.
- Bosch F.X., Muñoz N., de Sanjosé S., Izarzugaza I., Gili M., Viladiu P. et al. Risk factors for cervical cancer in Colombia and Spain. Int. J. Cancer. 1992;52(5):750-8. https://dx.doi.org/10.1002/ijc.2910520514.
- Cuzick J., Terry G., Ho L., Hollingworth T., Anderson M. Type-specific human papillomavirus DNA in abnormal scrapes as a predictor of high-grade cervical intraepithelial neoplasia. Br. J. Cancer 1994;69(1):167-71. https://dx.doi.org/10.1038/bjc.1994.28.
- Амирханян А.С., Прилепская В.Н., Байрамова Г.Р., Бурменская О.В., Костава М.Н., Асатурова А.В. Хронический цервицит: современные возможности диагностики и лечения. Акушерство и гинекология. 2018;4:22-7. [Amirkhanyan A.S., Prilepskaya V.N., Bairamova G.R., Burmenskaya O.V., Kostava M.N., Asaturova A.V. Chronic cervicitis: current opportunities for diagnosis and treatment. Obstetrics and Gynecology. 2018;(4):22-7. (in Russian)]. https://dx.doi.org/10.18565/aig.2018.4.22-27.
- Андреев А.О., Байрамова Г.Р., Зарецкий А.Р., Ребриков Д.В. Современные представления о ВПЧ как о мультифакторном предикторе развития плоскоклеточных интраэпителиальных поражений шейки матки. Акушерство и гинекология. 2022;11:60-6. [Andreev A.O., Bairamova G.R., Zaretsky A.R., Rebrikov D.V. Current concepts of HPV as a multifactorial predictor for squamous intraepithelial lesions of the cervix. Obstetrics and Gynecology. 2022;(11):60-6. (in Russian)]. https://dx.doi.org/10.18565/aig.2022.11.60-66.
- Lorincz A.T., Castle P.E., Sherman M.E., Scott D.R., Glass A.G., Wacholder S. et al. Viral load of human papillomavirus and risk of CIN3 or cervical cancer. Lancet. 2002;360(9328):228-9. https://dx.doi.org/10.1016/S0140-6736(02)09463-1
- Castle P.E., Schiffman M., Wheeler C.M. Hybrid capture 2 viral load and the 2-year cumulative risk of cervical intraepithelial neoplasia grade 3 or cancer. Am. J. Obstet. Gynecol. 2004;191(5):1590-7. https://dx.doi.org/10.1016/ j.ajog.2004.05.018.
- Castle P.E., Schiffman M., Scott D.R., Sherman M.E., Glass A.G., Rush B.B. et al. Semiquantitative human papillomavirus type 16 viral load and the prospective risk of cervical precancer and cancer. Cancer Epidemiol. Biomarkers Prev. 2005;14(5):1311-4. https://dx.doi.org/10.1158/1055-9965.EPI-04-0799
- Sherman M.E., Wang S.S., Wheeler C.M., Rich L., Gravitt P.E, Tarone R., Schiffman M. Determinants of human papillomavirus load among women with histological cervical intraepithelial neoplasia 3: dominant impact of surrounding low-grade lesions. Cancer Epidemiol. Biomarkers Prev. 2003;12(10):1038-44.
- Коган Е.А., Файзуллина Н.М., Ли Ц., Демура Т.А., Жарков Н.В., Козаченко А.В., Чернова В.Ф., Байрамова Г.Р., Прилепская В.Н. Ранняя диагностика ВПЧ-ассоциированной патологии шейки матки у женщин до 30 лет и старше. Акушерство и гинекология. 2015;9:62-7. [Kogan E.A., Faizullina N.M., Li Ts., Demura T.A., Zharkov N.V., Kozachenko A.V., Chernova V.F., Bairamova G.R., Prilepskaya V.N. Early diagnosis of HPV-associated disease of the cervix uteri in women aged less than 30 years or older. Obstetrics and Gynecology. 2015;(9):62-7. (in Russian)].
- Adcock R., Cuzick J., Hunt W.C., McDonald R.M., Wheeler C.M.; New Mexico HPV Pap Registry Steering Committee. Role of HPV genotype, multiple infections, and viral load on the risk of high-grade cervical neoplasia. Cancer Epidemiol. Biomarkers Prev. 2019;28(11):1816-24. https://dx.doi.org/10.1158/1055-9965.EPI-19-0239.
- Oyervides-Muñoz M.A., Pérez-Maya A.A., Sánchez-Domínguez C.N., Berlanga-Garza A., Antonio-Macedo M., Valdéz-Chapa L.D. et al. Multiple HPV infections and viral load association in persistent cervical lesions in Mexican women. Viruses. 2020;12(4):380. https://dx.doi.org/10.3390/v12040380.
- Zhou Y., Shi X., Liu J., Zhang L. Correlation between human papillomavirus viral load and cervical lesions classification: A review of current research. Front. Med. (Lausanne). 2023;10:1111269. https://dx.doi.org/10.3389/fmed.2023.1111269
- Андреев А.О., Байрамова Г.Р., Ильясова Н.А., Асатурова А.В., Трофимов Д.Ю. Ретроспективный анализ распространенности вируса папилломы человека у женщин с патологией шейки матки. Акушерство и гинекология. 2023;11:140-9. [Andreev A.O., Bayramova G.R., Ilyasova N.A., Asaturova A.V., Trofimov D.Yu. Retrospective analysis of human papillomavirus prevalence in women with cervical pathology. Obstetrics and Gynecology. 2023;(11):140-9. (in Russian)]. https://dx.doi.org/10.18565/aig.2023.225.
- Liu Y., Xu C., Pan J., Sun C., Zhou H., Meng Y. Significance of the viral load of high-risk HPV in the diagnosis and prediction of cervical lesions: a retrospective study. BMC Women’s Health. 2021;21(1):353. https://dx.doi.org/10.1186/s12905-021-01493-0.
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