Kaede Protein Chromophore Analogue as a Tool for Simultaneous Selective Staining of the Nucleus and Mitochondria

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Abstract

A series of three dyes – analogs of the chromophore of the fluorescent protein Kaede with various substituents – was synthesized. Based on fluorescence microscopy results, a promising fluorogen was identified: 5-((Z)-2-(difluoroboranyl)-4-(dimethylamino)-5-hydroxybenzylidene)-3-methyl-2-((E)-2-(pyridin-4-yl)-vinyl)-3,5-dihydro-4H-imidazol-4-one. The successful application of this dye in wide-field fluorescence microscopy for simultaneous selective staining of nuclei and mitochondria in living cells was demonstrated using the HeLa Kyoto cell line as an example. The synthesized compound may find application in the field of visualization of living system processes.

About the authors

D. I Rudik

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS

Moscow, Russia

A. R Gilvanov

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS; Pirogov Russian National Research Medical University

Moscow, Russia; Moscow, Russia

M. S Baranov

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS; Pirogov Russian National Research Medical University

Moscow, Russia; Moscow, Russia

Yu. A Bogdanova

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS; Pirogov Russian National Research Medical University

Email: zashiki.vvarashi@gmail.com
Moscow, Russia; Moscow, Russia

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