Assessment of Adaptive Immune Response Against Influenza Using Synthetic Peptides
- Authors: Gribovskaya O.V1, Tsygankov A.M2, Martinovich V.P1, Yanchenko V.V2
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Affiliations:
- Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus
- Vitebsk State medical University
- Issue: Vol 51, No 6 (2025)
- Pages: 1027-1037
- Section: ЭКСПЕРИМЕНТАЛЬНЫЕ СТАТЬИ
- URL: https://ogarev-online.ru/0132-3423/article/view/356308
- DOI: https://doi.org/10.7868/S1998286025060024
- ID: 356308
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Abstract
This study tested a method for assessing the immunogenicity of synthetic peptides, which could form the basis for a clinical diagnostic method for the cellular immune response to influenza A virus. The two peptides used are identical in amino acid composition to the 9-mer epitopes of influenza virus surface proteins relevant to the vaccine strains of the Northern Hemisphere for the 2023–2024 season and represent a fragment (432–440 aa) of influenza A virus hemagglutinin (Phe-Leu-Asp-Ile-Trp-Thr-Tyr-Asn-Ala) and a fragment (454–462 aa) of influenza B virus neuraminidase (Leu-Leu-Trp-Asp-Thr-Val-Thr-Gly-Val). The peptides were synthesized using classical methods of peptide chemistry, with activated esters and the carbodiimide method as the main condensation methods. Fifty-five volunteers aged 20–26 years participated in the clinical study. Gamma interferon (IFN-γ) levels were assessed by enzyme-linked immunosorbent assay. No statistically significant results were obtained for all pairwise comparisons of IFN-γ concentrations in 55 cases. This method needs to be optimized for clinical diagnosis of the cellular immune response to influenza A virus.
About the authors
O. V Gribovskaya
Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus
Email: olymelnik@yandex.ru
Minsk, Belarus
A. M Tsygankov
Vitebsk State medical UniversityVitebsk, Belarus
V. P Martinovich
Institute of Bioorganic Chemistry, National Academy of Sciences of BelarusMinsk
V. V Yanchenko
Vitebsk State medical UniversityVitebsk, Belarus
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