Development and Validation of GC-MS Bioanalytical Method to Detect Organic Acidemia in Neonatal/Pediatric Urine Samples
- Authors: Dhokade P.1, Mathew E.M.1, Nalini K.2, Rao P.2, Lewis L.3, Moorkoth S.1
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Affiliations:
- Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education
- Department of Biochemistry, Kasturba Medical College, Manipal Academy of Higher Education
- Department of Pediatrics, Kasturba Medical College, Manipal Academy of Higher Education
- Issue: Vol 52, No 6 (2018)
- Pages: 582-586
- Section: Article
- URL: https://ogarev-online.ru/0091-150X/article/view/245352
- DOI: https://doi.org/10.1007/s11094-018-1863-9
- ID: 245352
Cite item
Abstract
This work was aimed at developing an analytical tool for the simultaneous quantification of methylmalonic acid, glutaric acid, succinyl acetone, homogentisic acid, and pipecolic acid in pediatric urine samples. Organic acids were isolated from urine by liquid-liquid extraction. The extracted samples were then derivatized by using BSTFA + 1% TMCS solution, which resulted in the formation of trimethylsilyl ester derivatives. These analytes were quantified by GC-MS, and this bioanalytical method was validated according to the USFDA guidelines. The proposed method was applied to several clinically suspected organic acidemia samples. The linearity range is within 5 – 100 μg/mL for methylmalonic acid and glutaric acid, 20 – 100 μg/mL for succinyl acetone and homogentisic acid, and 10 – 100 μg/mL for pipecolic acid. Mean % recovery of QC samples of methylmalonic acid, glutaric acid, succinyl acetone, homogentisic acid, and pipecolic acid was found to be 92.06, 92.21, 90.92, 93.17, 90.71%, respectively, and that of tropic acid was 96.57%. All organic acids were stable at room temperature for 8 h. The stability of succinyl acetone, homogentisic acid, and pipecolic acid stored for 30 days at 8°C was found to be lower. The proposed method was applied to the analysis of samples obtained from 23 patients.
About the authors
Pallavi Dhokade
Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education
Email: sudheermoorkoth@gmail.com
India, Manipal, Karnataka, 576104
Elizabeth M. Mathew
Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education
Email: sudheermoorkoth@gmail.com
India, Manipal, Karnataka, 576104
K. Nalini
Department of Biochemistry, Kasturba Medical College, Manipal Academy of Higher Education
Email: sudheermoorkoth@gmail.com
India, Manipal, Karnataka, 576104
Pragna Rao
Department of Biochemistry, Kasturba Medical College, Manipal Academy of Higher Education
Email: sudheermoorkoth@gmail.com
India, Manipal, Karnataka, 576104
Leslie Lewis
Department of Pediatrics, Kasturba Medical College, Manipal Academy of Higher Education
Email: sudheermoorkoth@gmail.com
India, Manipal, Karnataka, 576104
Sudheer Moorkoth
Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education
Author for correspondence.
Email: sudheermoorkoth@gmail.com
India, Manipal, Karnataka, 576104
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