Отправить статью по E-mail Development and Validation of an HPLC-UV Method for Anilocaine Determination in Blood Plasma
- Авторы: Ryzhikova V.A.1, Kursakov S.V.2, Belov V.Y.1,2, Sevast’yanov V.I.1,2
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Учреждения:
- V. I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation
- Institute of Biomedical Research and Technology
- Выпуск: Том 52, № 2 (2018)
- Страницы: 166-170
- Раздел: Article
- URL: https://ogarev-online.ru/0091-150X/article/view/245051
- DOI: https://doi.org/10.1007/s11094-018-1784-7
- ID: 245051
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Аннотация
A method for anilocaine determination in rat blood plasma using HPLC with UV-detection was developed. Samples were prepared by liquid—liquid extraction with 1-BuOH—hexane (2:98, v/v) followed by stripping with aqueous formic acid (0.1%). Chromatographic analysis used a Nucleodur HILIC column eluted in isocratic mode by a mobile phase of MeCN and aqueous ammonium formate (25 mM) (85:15) and UV detection at 205 nm. The method was validated for selectivity, carry-over, calibration curve linearity, precision, accuracy, limit of quantitation, dilution integrity, and stability. The analytical range was 25 – 1,000 ng/mL. The proposed method could be applied to pharmacokinetic studies of anilocaine preparations.
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Об авторах
V. Ryzhikova
V. I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation
Email: chem@folium.ru
Россия, Moscow, 123182
S. Kursakov
Institute of Biomedical Research and Technology
Email: chem@folium.ru
Россия, Moscow, 123557
V. Belov
V. I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation; Institute of Biomedical Research and Technology
Email: chem@folium.ru
Россия, Moscow, 123182; Moscow, 123557
V. Sevast’yanov
V. I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation; Institute of Biomedical Research and Technology
Email: chem@folium.ru
Россия, Moscow, 123182; Moscow, 123557
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