Development of an HPLC-UV Method for Quantitative Determination of Acetylsalicylic Acid and Its Main Metabolite

详细

An HPLC-UV method for determination of acetylsalicylic acid and its main metabolite, salicylic acid, in a model solution and in rabbit blood plasma was developed. Plasma samples were prepared by salting out. Chromatographic analysis was performed in isocratic mode over a Hypersil BDS C18 column using mobile phase MeCN—H₂O (pH 2.5, 30:70) with detection at 230 nm. The limit of quantitation for acetylsalicylic and salicylic acids in the model solution was 0.05 μg/mL; in blood plasma, 0.2 μg/mL. The developed method was applied to the development of new acetylsalicylic-acid dosage forms based on biocompatible polymer carriers, including pharmacokinetic studies after i.m. implantation.