Selection of Optimum Parameters for Separating Amino Acids in a Thin Layer of Sorbent

Abstract

A theoretical approach to selecting the optimal conditions for separating various amino acids in thin sorbent layers is demonstrated, i.e., the ability to predict R_f values from the equations for linear relationships when the polarity of the eluant is known. For simultaneous assays, the optimum values of R_f and the best separations are obtained by working with systems in the range 5.13 – 5.69 polarity units. The relationships found in the study can be used to develop various methods which, depending on the aims of the analysis and the suspected amino acid composition, will find use in quality control of formulations containing both single and multiple amino acids and fourth-generation vitamin complexes, as well as for separating and identifying free amino acids present in the raw materials for medicinal herbs and phytopreparations.