Efficacy of human living skin equivalent in the treatment of inherited epidermolysis bullosa
- Authors: Karamova A.E.1, Kubanov A.A.1, Vorotelyak E.A.2, Rogovaya O.S.2, Chikin V.V.1, Nefedova M.A.1, Monchakovskaya E.S.1
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Affiliations:
- State Research Center of Dermatovenereology and Cosmetology
- N.K. Koltsov Institute of Developmental Biology, Russian Academy of Sciences
- Issue: Vol 99, No 6 (2023)
- Pages: 29-36
- Section: ORIGINAL STUDIES
- URL: https://ogarev-online.ru/0042-4609/article/view/252154
- DOI: https://doi.org/10.25208/vdv16249
- ID: 252154
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Abstract
Background. Inherited epidermolysis bullosa is a group of genetic skin disorders caused by mutations in genes encoding structural proteins of epidermis and dermo-epidermal junction. Clinical manifestations are characterized by spontaneous or trauma-induced skin and/or mucosal blistering, and extensive wounds. The use of tissue-engineered skin substitutes composed of allogeneic human skin cells is considered to be a perspective therapeutic approach in improving wound healing process.
Aims. To assess safety and efficacy of human skin equivalent in the treatment of inherited epidermolysis bullosa patients
Methods. 7 patients (5 female and 2 male subjects) from the age of 20 to 55 with inherited epidermolysis bullosa with different clinical subtypes were enrolled in the study: 3 patients with intermediate recessive dystrophic epidermolysis bullosa, 2 patients with severe recessive dystrophic epidermolysis bullosa, 1 patient with dominant dystrophic epidermolysis bullosa and 1 patient with junctional epidermolysis bullosa. Transplantation of composite allogeneic living skin equivalent comprising allogeneic keratinocytes and fibroblasts in low concentration (5 mg/ml) embedded within a type I collagen gel matrix was performed. The living skin equivalent was developed at N.K. Koltsov Institute of Developmental Biology. 19 erosions and ulcers with a surface area between 0.4 and 120 cm2 were evaluated. At day 14 clinical assessment was performed. To assess level of expression immunofluorescence antigen mapping was used.
Results. At day 14 complete erosion closure was achieved in 10 (53%) erosions. 4 (21%) erosions reduced in size > 75%. Size reduction between 25 and 75% was shown in a single (5%) case, no clinical efficacy was demonstrated in 4 (21%) cases. Collagen VII expression increased comparing to baseline level and accompanied clinical improvement.
Conclusions. The obtained data showed clinical efficacy of topical treatment with living skin equivalent, although no statistically significant difference was seen between living skin equivalent and atraumatic non-adhesive dressings.
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##article.viewOnOriginalSite##About the authors
Arfenya E. Karamova
State Research Center of Dermatovenereology and Cosmetology
Author for correspondence.
Email: karamova@cnikvi.ru
ORCID iD: 0000-0003-3805-8489
SPIN-code: 3604-6491
MD, Cand. Sci. (Med.), Assistant Professor
Russian Federation, 3 bldg 6 Korolenko street, 107076 MoscowAlexey A. Kubanov
State Research Center of Dermatovenereology and Cosmetology
Email: alex@cnikvi.ru
ORCID iD: 0000-0002-7625-0503
SPIN-code: 8771-4990
MD, Dr. Sci. (Med.), Professor, Academician of the Russian Academy of Sciences
Russian Federation, 3 bldg 6 Korolenko street, 107076 MoscowEkaterina A. Vorotelyak
N.K. Koltsov Institute of Developmental Biology, Russian Academy of Sciences
Email: vorotelyak@yandex.ru
ORCID iD: 0000-0001-5405-0212
SPIN-code: 2310-9118
Dr. Sci. (Biol.), Corresponding Member of the Russian Academy of Sciences
Russian Federation, MoscowOlga S. Rogovaya
N.K. Koltsov Institute of Developmental Biology, Russian Academy of Sciences
Email: rogovaya26f@gmail.com
ORCID iD: 0000-0003-4251-9372
SPIN-code: 1441-8532
Cand. Sci. (Biol.)
Russian Federation, MoscowVadim V. Chikin
State Research Center of Dermatovenereology and Cosmetology
Email: chikin@cnikvi.ru
ORCID iD: 0000-0002-9688-2727
SPIN-code: 3385-4723
MD, Dr. Sci. (Med.), Senior Researcher
Russian Federation, 3 bldg 6 Korolenko street, 107076 MoscowMariya A. Nefedova
State Research Center of Dermatovenereology and Cosmetology
Email: nefedova.maria.arb@gmail.com
ORCID iD: 0000-0003-1141-9352
Junior Research Associate
Russian Federation, 3 bldg 6 Korolenko street, 107076 MoscowEkaterina S. Monchakovskaya
State Research Center of Dermatovenereology and Cosmetology
Email: monchakovskaya@cnikvi.ru
ORCID iD: 0000-0002-6402-0962
SPIN-code: 9859-1912
Junior Research Associate
Russian Federation, 3 bldg 6 Korolenko street, 107076 MoscowReferences
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