Microbiological assessment of the effectiveness of standard therapy in atopic dermatitis
- Authors: Olisova O.Y.1, Svitich O.A.1,2, Poddubikov A.V.2, Vartanova N.O.2, Potapova M.B.1,2
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Affiliations:
- Sechenov University
- I. I. Mechnikov Research Institute of Vaccines and Sera
- Issue: Vol 99, No 3 (2023)
- Pages: 44-52
- Section: ORIGINAL STUDIES
- URL: https://ogarev-online.ru/0042-4609/article/view/131373
- DOI: https://doi.org/10.25208/vdv1364
- ID: 131373
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Abstract
Background. Atopic dermatitis is an inflammatory skin disease characterized by recurrent lesions and intense pruritus. Nowadays there is a stepwise approach to the treatment of atopic dermatitis, which is defined by disease intensity and complications such as secondary skin infections. However, the current management of atopic dermatitis may not always lead to the expected outcome due to not only immune dysregulation of both adaptive and innate immunity but also imbalance of the skin microbiome.
Aims. The aim of the study was to evaluate changes in the composition of the skin microbiome in both lesional and non-lesional skin in patients with atopic dermatitis during standard treatment.
Materials and methods. Twenty patients with atopic dermatitis and twenty six healthy controls over 18 years old were included into the study. All microbiome samples were obtained from lesional and non-lesional skin sites of atopic dermatitis patients before and after therapy. Whereas samples from healthy controls were taken once from a flexor surface of the elbow. Species identification of clinical isolates were identified using MALDI Biotyper Sirius (Bruker Daltonics).
Results. At baseline, the prevalence of S. aureus colonization among patients with atopic dermatitis was 34.20% in lesional skin and 32.50% in non-lesional skin. After treatment, there was a significant decrease in the prevalence of S. aureus carriage in both lesional and non-lesional skin areas (р < 0.05). However, no significant difference was observed in the proportion of all other staphylococci (р < 0.1). Interestingly, S. aureus was not found in healthy controls.
Conclusions. The results of the study demonstrated the effectiveness of standard therapy for managing patients with atopic dermatitis as it had a positive impact on the skin microbial community and showed a decrease in S. aureus proportion after the treatment.
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##article.viewOnOriginalSite##About the authors
Olga Yu. Olisova
Sechenov University
Email: olisovaolga@mail.ru
ORCID iD: 0000-0003-2482-1754
SPIN-code: 2500-7989
MD, Dr. Sci. (Med.), Professor, Corresponding Member of the Russian Academy of Sciences
Russian Federation, MoscowOxana A. Svitich
Sechenov University; I. I. Mechnikov Research Institute of Vaccines and Sera
Email: svitichoa@yandex.ru
ORCID iD: 0000-0003-1757-8389
SPIN-code: 8802-5569
MD, Dr. Sci. (Med.), Professor, Corresponding Member of the Russian Academy of Sciences
Russian Federation, Moscow; MoscowAlexander V. Poddubikov
I. I. Mechnikov Research Institute of Vaccines and Sera
Email: poddubikov@yandex.ru
ORCID iD: 0000-0001-8962-4765
SPIN-code: 9658-1553
MD, Cand. Sci. (Med.)
Russian Federation, MoscowNune O. Vartanova
I. I. Mechnikov Research Institute of Vaccines and Sera
Email: labmicr@mail.ru
ORCID iD: 0000-0002-6372-9910
SPIN-code: 6795-0835
Cand. Sci. (Biol.)
Russian Federation, MoscowMariia B. Potapova
Sechenov University; I. I. Mechnikov Research Institute of Vaccines and Sera
Author for correspondence.
Email: ptpv.msh@gmail.com
ORCID iD: 0000-0001-9647-1322
Postgraduate Student
Russian Federation, Moscow; MoscowReferences
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