Results of molecular and genetic research of genes ompA, ct135 and tarP C. trachomatis strains collected from patients suffering from urogenital chlamydial infection
- Authors: PLAHOVA KI1, KOZHUSHNAYA OS1, FRIGO NV1
-
Affiliations:
- ФГБУ «ГНЦДК» Минздравсоцразвития России
- Issue: Vol 88, No 4 (2012)
- Pages: 48-54
- Section: Articles
- URL: https://ogarev-online.ru/0042-4609/article/view/117174
- DOI: https://doi.org/10.25208/vdv702
- ID: 117174
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Abstract
The article contains results of the molecular and genetic research of genes ompA, ct135 and tarp c. trachomatis in 50 samples of clinical material containing DNA c. trachomatis from patients (women and men) from the Moscow region with the diagnosed urogenital chlamydial infection (UGCI) confirmed by PCR method. The sequencing of the gene ompA allowed to establish, that the prevailing serovar c. trachomatis in the examined selection of UGCI patients was serovar Е (48,0%); other serovars were less often (G — 18,0%, D — 14,0%; F and J — by 8%, К — 4%). The authors revealed authentic associations of the frequency of spreading of Е serovar with non-complicated clinical course of UGCI and the association of the frequency of spreading concerning rare serovars c. trachomatis (G, D, J) — with complications of UGCI in women. Significant mutations of the gene ompА in rare c. trachomatis serovars, аs well as significant mutations of genes ct135 and tarр were revealed more often is samples from women with PID. The obtained data are sufficient to make a conclusion about the possible existing relation between peculiarities of c. trachomatis genotype and the character of the clinical course of the urogenital clamidiosis.
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##article.viewOnOriginalSite##About the authors
K I PLAHOVA
ФГБУ «ГНЦДК» Минздравсоцразвития России
Email: plahova_xenia@mail.ru
к.м.н., старший научный сотрудник отдела ИППП
O S KOZHUSHNAYA
ФГБУ «ГНЦДК» Минздравсоцразвития Россиимладший научный сотрудник отдела ИППП
N V FRIGO
ФГБУ «ГНЦДК» Минздравсоцразвития Россиид.м.н., заместитель директора по научно-образовательной работе
References
- Данные ВОЗ. WHO. Infections with a predominantly sexual mode of transmission, Certain Infectious and Parasitic Diseases, 2011. Availabe at: http://www.who.int/.
- Генетический паспорт — основа индивидуальной и предиктивной медицины/ Под ред. В.С. Баранова. СПб: Изд-во Н-Л 2009; 528 с: илл.
- Geisler W.M., Suchland R.J., Whittington W.L., Stamm W.E. The relationship of serovar to clinical manifestations of urogenital Chlamydia trachomatis infection. Sex Transm Dis 2003; 30(2): 160—5.
- Morré S.A., Rozendaal L., Van Valkengoed I.G., Boeke A., Van Voorst Vader P.C., Schirm J., de Blok S., Van Den Hoek J.A., Van Doornum G.J., Meijer C.J., Van Den Brule A.J. Urogenital Chlamydia trachomatis serovars in men and women with a symptomatic or asymptomatic infection: an association with clinical manifestations? J Clin Microbiol 2000; 38(6): 2292—6.
- Dean D., Bruno W.J., Wan R., Gomes J.P., Devignot S., Mehari T. at al. Predicting phenotype and emerging strains among Chlamydia trachomatis infections. J Bacteriol. 2004; 186 (13): 4295—11.
- Jalal H., Verlander N.Q., Kumar N., Bentley N., Carne C., Sonnex C. Genital chlamydial infection: association between clinical features, organism genotype and load. J Med Microbiol. 2011 Jul; 60(Pt 7): 881—8. Epub 2011 Mar 17.
- Hatch T.P., Vance D.W., Al-Hossainy Y. Identification of a major envelope proeine in Chlamydia spp. 1981. J Bacteriol, 146, 426—429.
- Lutter Erika I., Bonner Christine, Holland Martin J., Suchland Robert J., Stamm Walter E., Jewett Travis J., McClarty Grant, Hackstadt Ted. Phylogenetic Analysis of Chlamydia trachomatis Tarp and Correlation with Clinical Phenotype. Infect. Immun. September 2010 vol. 78 no. 9 3678—3688.
- Brunelle G.W., Sensabough G.F. The ompA gene in Chlamydia trachomatis differs in phylogeny and rate of evolution from other regions of the genome. Infect Immun 2006; 74: 578—585.
- Spaaragen J., Verhaest I., Mooi J.S. et al. Analysis of Chlamydia trachomatis. Serovar distribution changes in the Netherlands (1986—2002). Sex Transm Infect 2005; 80: 151—152.
- Lister N.A., Faitley C.K.C. trachomatis serovars causing urogenital infections in women in Melbourne, Australia. J Clin Microbiol. 2005; 43: 2546—2547.
- Jurstrand M., Falk L., Fredlund H., Lindberg M., Olcén P., Andersson S., Persson K., Albert J., and Bäckman A. Characterization of Chlamydia trachomatis omp1 Genotypes among Sexually Transmitted Disease Patients in Sweden. J Clin Microbiol 2001; 39 (11): 3915—3919.
- Mossman D., Beagley K.W., Landay A.L., Loewenthal M., Ooi C., Timms P., Boyle M. Genotyping of Urogenital Chlamydia trachomatis in Regional New South Wales, Australia. Sex Trans Dis 2008; 35 (6): 614—4.
- Millman K., Black C.M., Johnson R.E., Stamm W.E., Jones R.B., Hook E.W., Martin D.H., Bolan G., Tavare S., Dean D. Population-based genetic and evolutionary analysis of Chlamydia trachomatis urogenital strain variation in the United States, J Bacteriol 2004; 2457—9.
- Lister N.A., Faitley C.K.C. trachomatis serovars causing urogenital infections in women in Melbourne, Australia. J Clin Microbiol 2005; 43: 2546—2547.
- Jurstrand M., Falk L., Fredlund H., Lindberg M., Olcén P., Andersson S., Persson K., Albert J., Bäckman A. Characterization of Chlamydia trachomatis omp1 Genotypes among Sexually Transmitted Disease Patients in Sweden. J Clin Microbiol 2001; 39 (11): 3915—3919.
- Sturdevant G.L., Kari L., Gardner D.J., Olivares-Zavaleta N., Randall L.B., Whitmire W.M., Carlson J.H., Goheen M.M., Selleck E.M., Martens C., Caldwel H.D. Frameshift Mutations in a Single Novel Virulence Factor Alter the In Vivo Pathogenicity of Chlamydia trachomatis for the Female Murine Genital Tract Infect Immun. 2010 September; 78(9): 3660—3668.
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