Optical Detection of Immune Complexes Using Colloidal Gold Nanoparticles


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Аннотация

The possibility of detecting the formation of immune complexes with the use of antibodies labeled with colloidal gold nanoparticles and optical spectroscopy is considered. Changes in the spectral characteristics of sols of gold nanoparticles with diameters of 15, 60, and 90 nm conjugated with immunoglobulins in the presence of especially dangerous infectious agents are recorded and analyzed. The data obtained indicate that the developed methodological approach to optical detection of immune complexes may be promising for creating immunochemical methods of identification of infectious agents.

Авторлар туралы

A. Spitsyn

Russian Antiplague Research Institute Microbe

Хат алмасуға жауапты Автор.
Email: rusrapi@microbe.ru
Ресей, Saratov, 410005

D. Utkin

Russian Antiplague Research Institute Microbe

Email: rusrapi@microbe.ru
Ресей, Saratov, 410005

M. Kireev

Russian Antiplague Research Institute Microbe

Email: rusrapi@microbe.ru
Ресей, Saratov, 410005

N. Sharapova

Russian Antiplague Research Institute Microbe

Email: rusrapi@microbe.ru
Ресей, Saratov, 410005

P. Erokhin

Russian Antiplague Research Institute Microbe

Email: rusrapi@microbe.ru
Ресей, Saratov, 410005

V. Germanchuk

Russian Antiplague Research Institute Microbe

Email: rusrapi@microbe.ru
Ресей, Saratov, 410005

V. Kochubey

Chernyshevsky Saratov National Research State University; National Research Tomsk State University

Email: rusrapi@microbe.ru
Ресей, Saratov, 410012; Tomsk, 634050

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