Terahertz Microscope Based on Solid Immersion Effect for Imaging of Biological Tissues
- Authors: Chernomyrdin N.V.1,2, Kucheryavenko A.S.1,2, Rimskaya E.N.2, Dolganova I.N.2,3, Zhelnov V.A.2, Karalkin P.A.4,5, Gryadunova A.A.4,6, Reshetov I.V.6, Lavrukhin D.V.7, Ponomarev D.S.7, Karasik V.E.2, Zaytsev K.I.1,2
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Affiliations:
- Prokhorov General Physics Institute, Russian Academy of Sciences
- Bauman Moscow State Technical University
- Institute of Solid-State Physics, Russian Academy of Sciences
- 3D Bioprinting Solutions
- National Medical Research Center of Radiology
- Institute of Regenerative Medicine, Sechenov First Moscow State Medical University
- Institute of Ultra High Frequency Semiconductor Electronics, Russian Academy of Sciences
- Issue: Vol 126, No 5 (2019)
- Pages: 560-567
- Section: Biophotonics
- URL: https://ogarev-online.ru/0030-400X/article/view/165994
- DOI: https://doi.org/10.1134/S0030400X19050059
- ID: 165994
Cite item
Abstract
We propose a new method of terahertz microscopy for imaging of biological tissues with a subwavelength spatial resolution. It makes it possible to surmount the Abbe diffraction limit and ensures a subwavelength resolution due to the solid immersion effect, i.e., due to decreasing dimensions of the electromagnetic beam caustic as the beam is focused in free space at a small distance (smaller than the wavelength) behind a medium with a high refractive index. An experimental setup that realizes the proposed method is developed. It uses a backward-wave oscillator and a Golay cell as a source and a detector of the terahertz radiation, respectively. In this setup, the radiation is focused behind a silicon hemisphere to realize the solid immersion effect. A record-high spatial resolution of 0.15λ is demonstrated experimentally for optical systems based on the solid immersion effect (the measurements have been performed at a wavelength of λ = 500 μm using a metal–air interface as a test object). Microscopy based on the solid immersion effect does not imply using diaphragms or near-field probes of other types for achieving the subwavelength spatial resolution, and, correspondingly eliminates energy losses associated with these elements. The proposed method has been applied for imaging of soft biological tissues, which has made it possible to demonstrate its potential for the use in biology and medicine.
About the authors
N. V. Chernomyrdin
Prokhorov General Physics Institute, Russian Academy of Sciences; Bauman Moscow State Technical University
Author for correspondence.
Email: chernik-a@yandex.ru
Russian Federation, Moscow, 119991; Moscow, 105005
A. S. Kucheryavenko
Prokhorov General Physics Institute, Russian Academy of Sciences; Bauman Moscow State Technical University
Email: chernik-a@yandex.ru
Russian Federation, Moscow, 119991; Moscow, 105005
E. N. Rimskaya
Bauman Moscow State Technical University
Email: chernik-a@yandex.ru
Russian Federation, Moscow, 105005
I. N. Dolganova
Bauman Moscow State Technical University; Institute of Solid-State Physics, Russian Academy of Sciences
Email: chernik-a@yandex.ru
Russian Federation, Moscow, 105005; Chernogolovka, 142432
V. A. Zhelnov
Bauman Moscow State Technical University
Email: chernik-a@yandex.ru
Russian Federation, Moscow, 105005
P. A. Karalkin
3D Bioprinting Solutions; National Medical Research Center of Radiology
Email: chernik-a@yandex.ru
Russian Federation, Moscow, 115409; Moscow, 125284
A. A. Gryadunova
3D Bioprinting Solutions; Institute of Regenerative Medicine, Sechenov First Moscow State Medical University
Email: chernik-a@yandex.ru
Russian Federation, Moscow, 115409; Moscow, 119991
I. V. Reshetov
Institute of Regenerative Medicine, Sechenov First Moscow State Medical University
Email: chernik-a@yandex.ru
Russian Federation, Moscow, 119991
D. V. Lavrukhin
Institute of Ultra High Frequency Semiconductor Electronics, Russian Academy of Sciences
Email: chernik-a@yandex.ru
Russian Federation, Moscow, 117105
D. S. Ponomarev
Institute of Ultra High Frequency Semiconductor Electronics, Russian Academy of Sciences
Email: chernik-a@yandex.ru
Russian Federation, Moscow, 117105
V. E. Karasik
Bauman Moscow State Technical University
Email: chernik-a@yandex.ru
Russian Federation, Moscow, 105005
K. I. Zaytsev
Prokhorov General Physics Institute, Russian Academy of Sciences; Bauman Moscow State Technical University
Email: chernik-a@yandex.ru
Russian Federation, Moscow, 119991; Moscow, 105005
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