Femtosecond Absorption Spectroscopy of Reduced and Oxidized Forms of Cytochrome c Oxidase: Excited States and Relaxation Processes in Heme a and a3 Centers


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Abstract

Excited electronic states and intraheme relaxation processes in the oxidized and reduced forms of mitochondrial cytochrome c oxidase extracted from a beef heart have been investigated by femtosecond absorption spectroscopy. The spectral and kinetic characteristics of short-lived intermediates have been measured from 80 fs to 20 ps after the photoexcitation. It is found that nonradiative electronic relaxation of the excitation energy in heme a, both in the oxidized (Fe(III)a) and reduced (Fe(II)a) forms, occurs successively as three processes, after the end of which heme a is in the ground state with a large store of vibrational energy. The subsequent vibrational relaxation (heme cooling) lasts for several picoseconds. It is found for reduced heme a3 (Fe(II)a3) that the electronic relaxation occurs as a result of two successive stages, which changes to vibrational relaxation in the ground state. The mechanism and dynamics of electronic excitation energy conversion in cytochrome c oxidase are analyzed.

About the authors

I. V. Shelaev

Semenov Institute of Chemical Physics, Russian Academy of Sciences

Email: b.dzhagarov@ifanbel.bas-net.by
Russian Federation, Moscow, 119991

F. E. Gostev

Semenov Institute of Chemical Physics, Russian Academy of Sciences

Email: b.dzhagarov@ifanbel.bas-net.by
Russian Federation, Moscow, 119991

T. V. Vygodina

Belozerski Institute of Physicochemical Biology, Moscow State University

Email: b.dzhagarov@ifanbel.bas-net.by
Russian Federation, Moscow, 119991

S. V. Lepeshkevich

Stepanov Institute of Physics, National Academy of Sciences of Belarus

Email: b.dzhagarov@ifanbel.bas-net.by
Belarus, Minsk, 220072

B. M. Dzhagarov

Stepanov Institute of Physics, National Academy of Sciences of Belarus

Author for correspondence.
Email: b.dzhagarov@ifanbel.bas-net.by
Belarus, Minsk, 220072

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