Email this article An Acousto-Optic Hyperspectral Unit for Histological Study of Microscopic Objects
- Authors: Polschikova O.V.1, Machikhin A.S.1,2, Ramazanova A.G.1, Bratchenko I.A.3, Pozhar V.E.1, Danilycheva I.V.4,5, Katunina O.R.6, Danilychev M.V.7
-
Affiliations:
- Scientific and Technological Center of Unique Instrumentation, Russian Academy of Sciences
- National Research University Moscow Power Engineering Institute (Technical University)
- Korolyov Samara State Aerospace University (National Research University)
- National Research Center Institute of Immunology of the Federal Medical-Biological Agency of Russia
- Evdokimov Moscow State University of Medicine and Dentistry
- State Research Center Burnasyan Federal Medical Biophysical Center
- Kotelnikov Institute of Radio Engineering and Electronics, Russian Academy of Sciences
- Issue: Vol 125, No 6 (2018)
- Pages: 1074-1080
- Section: Applied Optics
- URL: https://ogarev-online.ru/0030-400X/article/view/165905
- DOI: https://doi.org/10.1134/S0030400X19020188
- ID: 165905
Cite item
Open Access
Access granted
Subscription Access
Abstract
The problem of the development of an adjustable video-spectrometer unit based on an acousto-optic tunable filter has been considered. This unit is embedded into an optical system of microscopes for hyperspectral analysis of microscopic objects. For this purpose, light–energy conjugation of the filter with microscopes is performed. We have calculated the conjugating optical system, which yields an equality of field of view for a wideband and spectral channel. The experimental model of the developed device has been described. Examples of detection for test objects and histological sections along with the calculation results of the spectral parameters for various skin tissues are given. The developed video-spectrometer unit considerably extends the functionality of microscopes and can be widely used to solve various problems of biomedical diagnostics.
About the authors
O. V. Polschikova
Scientific and Technological Center of Unique Instrumentation, Russian Academy of Sciences
Author for correspondence.
Email: polschikova@gmail.com
Russian Federation, Moscow, 117342
A. S. Machikhin
Scientific and Technological Center of Unique Instrumentation, Russian Academy of Sciences; National Research University Moscow Power Engineering Institute (Technical University)
Email: polschikova@gmail.com
Russian Federation, Moscow, 117342; Moscow, 111250
A. G. Ramazanova
Scientific and Technological Center of Unique Instrumentation, Russian Academy of Sciences
Email: polschikova@gmail.com
Russian Federation, Moscow, 117342
I. A. Bratchenko
Korolyov Samara State Aerospace University (National Research University)
Email: polschikova@gmail.com
Russian Federation, Samara, 443086
V. E. Pozhar
Scientific and Technological Center of Unique Instrumentation, Russian Academy of Sciences
Email: polschikova@gmail.com
Russian Federation, Moscow, 117342
I. V. Danilycheva
National Research Center Institute of Immunology of the Federal Medical-Biological Agency of Russia; Evdokimov Moscow State University of Medicine and Dentistry
Email: polschikova@gmail.com
Russian Federation, Moscow, 115478; Moscow, 127473
O. R. Katunina
State Research Center Burnasyan Federal Medical Biophysical Center
Email: polschikova@gmail.com
Russian Federation, Moscow, 123098
M. V. Danilychev
Kotelnikov Institute of Radio Engineering and Electronics, Russian Academy of Sciences
Email: polschikova@gmail.com
Russian Federation, Moscow, 125009
Supplementary files
