Local tomographic phase microscopy from differential projections


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It is proposed to use local tomography for optical studies of the internal structure of transparent phase microscopic objects, for example, living cells. From among the many local tomography methods that exist, the algorithms of back projection summation (in which partial derivatives of projections are used as projection data) are chosen. The application of local tomography to living cells is reasonable because, using optical phase microscopy, one can easily obtain projection data in the form of first-order derivatives of projections applying the methods of differential interference contrast and shear interferometry. The mathematical fundamentals of local tomography in differential projections are considered, and a computer simulation of different local tomography methods is performed. A tomographic phase microscope and the results of reconstructing a local tomogram of an erythrocyte from a set of experimental differential projections are described.

作者简介

G. Vishnyakov

All-Russian Research Institute for Optical and Physical Measurements VNIIOFI

编辑信件的主要联系方式.
Email: vish@vniiofi.ru
俄罗斯联邦, Moscow, 119361

G. Levin

All-Russian Research Institute for Optical and Physical Measurements VNIIOFI

Email: vish@vniiofi.ru
俄罗斯联邦, Moscow, 119361

V. Minaev

All-Russian Research Institute for Optical and Physical Measurements VNIIOFI

Email: vish@vniiofi.ru
俄罗斯联邦, Moscow, 119361

N. Nekrasov

All-Russian Research Institute for Optical and Physical Measurements VNIIOFI

Email: vish@vniiofi.ru
俄罗斯联邦, Moscow, 119361

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