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Expression of heterologous PEP-carboxylase in Methylococcus capsulatus MIR: the influence on growth characteristics and amino acid composition of methanotrophic biomass
- Authors: But S.Y.1,2, Rozova O.N.1,2, Chistyakova S.V.2, Potapova D.S.3, Khmelenina V.N.2, Mustakhimov I.I.1,2
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Affiliations:
- Research Center of Biotechnology, Russian Academy of Sciences
- Pushchino Scientific Center for Biological Research, Russian Academy of Sciences
- Tula State University
- Issue: Vol 93, No 6 (2024)
- Pages: 879-883
- Section: SHORT COMMUNICATIONS
- URL: https://ogarev-online.ru/0026-3656/article/view/276185
- DOI: https://doi.org/10.31857/S0026365624060173
- ID: 276185
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Abstract
Methylococcus capsulatus MIR is a strain of methanotrophic bacteria that is potentially suitable for producing feed protein and other value-added products from methane. Genomic analysis did not reveal in Mc. capsulatus MIR known pathways of C3-carboxylation, necessary for the replenishment of intermediates of the tricarboxylic acid cycle and the full functioning of metabolism. The pepc gene encoding PEP carboxylase in Methylomonas rapida 12 was introduced into Mc. capsulatus MIR cells on a plasmid under the control of a medium-strength promoter. Expression of heterologous PEP carboxylase led to an increase in the content of glutamate, glycine and lysine in the cells of the recombinant strain, but did not increase to the growth rate of the culture. Consequently, the introduction of heterologous PEP carboxylase, which carries out the C3-carboxylation reaction, helps to increase the nutritional value of the methanotroph biomass.
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About the authors
S. Y. But
Research Center of Biotechnology, Russian Academy of Sciences; Pushchino Scientific Center for Biological Research, Russian Academy of Sciences
Author for correspondence.
Email: sergeybut20063@gmail.com
Winogradsky Institute of Microbiology; Institute of Biochemistry and Physiology of Microorganisms
Russian Federation, Moscow, 119071; Moscow region, Pushchino, 142290O. N. Rozova
Research Center of Biotechnology, Russian Academy of Sciences; Pushchino Scientific Center for Biological Research, Russian Academy of Sciences
Email: sergeybut20063@gmail.com
Winogradsky Institute of Microbiology; Institute of Biochemistry and Physiology of Microorganisms
Russian Federation, Moscow, 119071; Moscow region, Pushchino, 142290S. V. Chistyakova
Pushchino Scientific Center for Biological Research, Russian Academy of Sciences
Email: sergeybut20063@gmail.com
Institute of Biochemistry and Physiology of Microorganisms
Russian Federation, Moscow region, Pushchino, 142290D. S. Potapova
Tula State University
Email: sergeybut20063@gmail.com
Russian Federation, Tula, 300012
V. N. Khmelenina
Pushchino Scientific Center for Biological Research, Russian Academy of Sciences
Email: sergeybut20063@gmail.com
Institute of Biochemistry and Physiology of Microorganisms
Russian Federation, Moscow region, Pushchino, 142290I. I. Mustakhimov
Research Center of Biotechnology, Russian Academy of Sciences; Pushchino Scientific Center for Biological Research, Russian Academy of Sciences
Email: sergeybut20063@gmail.com
Winogradsky Institute of Microbiology; Institute of Biochemistry and Physiology of Microorganisms
Russian Federation, Moscow, 119071; Moscow region, Pushchino, 142290References
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