Мақаланы E-mail арқылы жіберу Application of the bacterial C-DAG system to analyze the ability of amyloids to seed protein aggregation in vitro
- Авторлар: Trubitsina N.P.1, Zemlyanko O.M.1, Zhouravleva G.A.1, Bondarev S.A.1
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Мекемелер:
- Saint Petersburg State University
- Шығарылым: Том 93, № 5 (2024)
- Беттер: 662-665
- Бөлім: SHORT COMMUNICATIONS
- URL: https://ogarev-online.ru/0026-3656/article/view/273140
- DOI: https://doi.org/10.31857/S0026365624050156
- ID: 273140
Дәйексөз келтіру
Ашық рұқсат
Рұқсат берілді
Аннотация
The search for new amyloid proteins, as well as the study of their properties, is an actual task, which can be solved by a number of different model systems. One of the most popular is the C-DAG approach. It is based on the analysis of aggregation of the investigated proteins on the surface of Escherichia coli cells. According to the original protocol, it can be used to demonstrate one of the characteristic properties of amyloids: the ability to bind the amyloid-specific dye Congo red and demonstrate apple-green birefringence. In addition, the C-DAG technique allows one to analyze the morphology of aggregates and their resistance to detergents. In this work, we tested using Sup35NM as an example whether aggregates on the surface of bacterial cells can act as inducers of aggregation of the corresponding protein.
Негізгі сөздер
Толық мәтін
Авторлар туралы
N. Trubitsina
Saint Petersburg State University
Email: s.bondarev@spbu.ru
Department of Genetics and Biotechnology
Ресей, 1199034, Saint PetersburgO. Zemlyanko
Saint Petersburg State University
Email: stanislavspbgu@gmail.com
Department of Genetics and Biotechnology, Laboratory of Amyloid Biology
Ресей, 1199034, Saint PetersburgG. Zhouravleva
Saint Petersburg State University
Email: s.bondarev@spbu.ru
Department of Genetics and Biotechnology, Laboratory of Amyloid Biology
Ресей, 1199034, Saint PetersburgS. Bondarev
Saint Petersburg State University
Хат алмасуға жауапты Автор.
Email: stanislavspbgu@gmail.com
Department of Genetics and Biotechnology, Laboratory of Amyloid Biology
Ресей, 1199034, Saint PetersburgӘдебиет тізімі
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