EFFECT OF SOLVENT ISOTOPE COMPOSITION (H2O AND D2O) ON LYSOZYME OLIGOMER FORMATION UNDER CRYSTALLIZATION CONDITIONS

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Abstract

Using the method of small-angle X-ray scattering (SAXS), the temperature dependencies (in the range of 4 to 30°C with a 1°C step) of the structure of a lysozyme solution during the growth of tetragonal crystals were obtained for H2O and D2O solvents. It was found that, regardless of the solvent type, dimers and octamers form in the lysozyme crystallization solution. The volume fractions of the oligomers (dimers and octamers) are inversely proportional to the temperature change, both in the case of the H2O solvent and the D2O solvent. Under identical temperature conditions, the volume fraction of oligomers in the lysozyme crystallization solution in D2O is approximately 8% higher than in H2O. However, the same oligomer content in the lysozyme crystallization solutions in H2O and D2O is achieved when the temperature of the H2O solution is approximately 10°C lower than that of the D2O solution. This may be explained by the distinct influence of the solvent on protein hydration.

About the authors

M. A Marchenkova

National Research Centre "Kurchatov Institute"; Southern Federal University

Email: marchenkova@crys.ras.ru
Moscow, Russia; Rostov-on-Don, Russia

P. V Konarev

National Research Centre "Kurchatov Institute"; Southern Federal University

Moscow, Russia; Rostov-on-Don, Russia

Yu. V Pisarevsky

National Research Centre "Kurchatov Institute"

Moscow, Russia

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