Experimental setup for studying dynamics of the calcium interaction in cells


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Abstract

A calcium cell signaling system is one of the first, which were formed in the course of evolution of systems. The understanding of calcium binding–uncaging dynamics is crucial in studies of corresponding intracellular processes. By now, a great number of calcium-dependent processes have been investigated. However, works that fully consider these processes are absent. This is specified in many respects by the instrumental abilities. In this work, requirements for the experimental setup intended for comprehensive studies of calcium interaction dynamics are briefly formulated, its block diagram is described, and the results of test experiments are presented.

About the authors

E. Yu. Loktionov

Bauman Moscow State Technical University

Author for correspondence.
Email: stcpe@bmstu.ru
Russian Federation, ul. Vtoraya Baumanskaya 5, str. 1, Moscow, 105005

M. G. Mikhaylova

UKE Center for Molecular Neurobiology Hamburg (ZMNH/UKE)

Email: stcpe@bmstu.ru
Germany, Falkenried 94, Hamburg, D-20251

D. S. Sitnikov

Joint Institute for High Temperatures

Email: stcpe@bmstu.ru
Russian Federation, ul. Izhorskaya 13, str. 2, Moscow, 125412

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