Отправить статью по E-mail Human Umbilical Cord Mesenchymal Stromal Cell-Derived Microvesicles Express Surface Markers Identical to the Phenotype of Parental Cells
- Авторы: Romanov Y.A.1,2, Volgina N.E.3, Dugina T.N.2, Kabaeva N.V.1, Sukhikh G.T.3
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Учреждения:
- National Medical Research Center for Cardiology, Ministry of Health of the Russian Federation
- CryoCenter Cord Blood Bank
- V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
- Выпуск: Том 166, № 1 (2018)
- Страницы: 124-129
- Раздел: Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
- URL: https://ogarev-online.ru/0007-4888/article/view/240866
- DOI: https://doi.org/10.1007/s10517-018-4300-x
- ID: 240866
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Аннотация
Production of microvesicles in culture of human umbilical cord multipotent mesenchymal stromal cells was studied and comparative analysis of the expression of some surface molecules (clusters of differentiation, CD) was performed. It was found that the mesenchymal stromal cells produce microvesicles in the amount sufficient for their detection by flow cytometry. Parallel analysis of the phenotypes of maternal mesenchymal stromal cells and secreted microvesicles revealed identical expression of surface molecules CD13, CD29, CD44, CD54, CD71, CD73, CD90, CD105, CD106, and HLA-I. The concentration of microvesicles in the conditioned medium was 17.9±4.6×106/ml; i.e. one cell produced ~40-50 (44.7±11.5) microvesicles over 2 days in culture.
Об авторах
Yu. Romanov
National Medical Research Center for Cardiology, Ministry of Health of the Russian Federation; CryoCenter Cord Blood Bank
Автор, ответственный за переписку.
Email: romanov@cryocenter.ru
Россия, Moscow; Moscow
N. Volgina
V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: romanov@cryocenter.ru
Россия, Moscow
T. Dugina
CryoCenter Cord Blood Bank
Email: romanov@cryocenter.ru
Россия, Moscow
N. Kabaeva
National Medical Research Center for Cardiology, Ministry of Health of the Russian Federation
Email: romanov@cryocenter.ru
Россия, Moscow
G. Sukhikh
V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: romanov@cryocenter.ru
Россия, Moscow
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