Formation of Tissue-Engineered Construct of Human Cartilage Tissue in a Flow-Through Bioreactor
- Авторы: Sevastianov V.I.1, Basok Y.B.1, Grigor’ev A.M.1, Kirsanova L.A.1, Vasilets V.N.1
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Учреждения:
- V. I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation
- Выпуск: Том 164, № 2 (2017)
- Страницы: 269-273
- Раздел: Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
- URL: https://ogarev-online.ru/0007-4888/article/view/239548
- DOI: https://doi.org/10.1007/s10517-017-3971-z
- ID: 239548
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Аннотация
We performed culturing of a cell-engineered construct of human cartilage tissue consisting of biopolymer microstructured collagen-containing hydrogel, human adipose tissue mesenchymal stromal cells, and induction chondrogenic culture medium in a specially designed flow-through bioreactor. On day 16 of the experiment, human adipose tissue mesenchymal stromal cells acquired flattened shape typical for chondroblasts, demonstrated high proliferative activity, and formed extracellular matrix. The observed histological changes in the cultured system attested to the beginning of the formation of a tissue-engineered construct of human cartilage tissue.
Об авторах
V. Sevastianov
V. I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation
Автор, ответственный за переписку.
Email: viksev@yandex.ru
Россия, Moscow
Yu. Basok
V. I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation
Email: viksev@yandex.ru
Россия, Moscow
A. Grigor’ev
V. I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation
Email: viksev@yandex.ru
Россия, Moscow
L. Kirsanova
V. I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation
Email: viksev@yandex.ru
Россия, Moscow
V. Vasilets
V. I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation
Email: viksev@yandex.ru
Россия, Moscow
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