Отправить статью по E-mail Visualization of Mesenchymal Stromal Cells in 2Dand 3D-Cultures by Scanning Electron Microscopy with Lanthanide Contrasting
- Авторы: Novikov I.A.1, Vakhrushev I.V.2, Antonov E.N.3, Yarygin K.N.2, Subbot A.M.1
-
Учреждения:
- Institute of Eye Diseases
- V. N. Orekhovich Research Institute of Biomedical Chemistry
- Institute on Laser and Information Technologies, Russian Academy of Sciences
- Выпуск: Том 162, № 4 (2017)
- Страницы: 558-562
- Раздел: Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
- URL: https://ogarev-online.ru/0007-4888/article/view/238373
- DOI: https://doi.org/10.1007/s10517-017-3659-4
- ID: 238373
Цитировать
Открытый доступ
Доступ предоставлен
Только для подписчиков
Аннотация
Mesenchymal stromal cells from deciduous teeth in 2D- and 3D-cultures on culture plastic, silicate glass, porous polystyrene, and experimental polylactoglycolide matrices were visualized by scanning electron microscopy with lanthanide contrasting. Supravital staining of cell cultures with a lanthanide-based dye (neodymium chloride) preserved normal cell morphology and allowed assessment of the matrix properties of the carriers. The developed approach can be used for the development of biomaterials for tissue engineering.
Об авторах
I. Novikov
Institute of Eye Diseases
Email: kletkagb@gmail.com
Россия, Moscow
I. Vakhrushev
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: kletkagb@gmail.com
Россия, Moscow
E. Antonov
Institute on Laser and Information Technologies, Russian Academy of Sciences
Email: kletkagb@gmail.com
Россия, Moscow
K. Yarygin
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: kletkagb@gmail.com
Россия, Moscow
A. Subbot
Institute of Eye Diseases
Автор, ответственный за переписку.
Email: kletkagb@gmail.com
Россия, Moscow
Дополнительные файлы
