Отправить статью по E-mail Atomic force microscopy-based single virus particle spectroscopy
- Авторы: Korneev D.V.1, Popova A.V.2,3, Generalov V.M.1, Zaitsev B.N.1
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Учреждения:
- Vector State Research Center of Virology and Biotechnology
- Moscow Institute of Physics and Technology (State University)
- State Research Center of Applied Microbiology and Biotechnology
- Выпуск: Том 61, № 3 (2016)
- Страницы: 413-419
- Раздел: Cell Biophysics
- URL: https://ogarev-online.ru/0006-3509/article/view/151994
- DOI: https://doi.org/10.1134/S0006350916030088
- ID: 151994
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Аннотация
Atomic force microscopy-based single virus particle force spectroscopy was developed using dielectrophoresis for fixing virions at the tip of an atomic force microscope (AFM) probe. Electron microscopic visualization was found to be necessary to prove the deposition of virus particles on the tip of the AFM probe, while fixation of single virions by incubating the tip with a virus suspension proved impossible. Force spectroscopy measurements were performed for the vaccinia virus, influenza virus, and bacteriophage AP22. ForceReader special software was designed for analyzing the force–distance curves.
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Об авторах
D. Korneev
Vector State Research Center of Virology and Biotechnology
Автор, ответственный за переписку.
Email: korneev_dv@vector.nsc.ru
Россия, Kol’tsovo, Novosibirsk region, 630559
A. Popova
Moscow Institute of Physics and Technology (State University); State Research Center of Applied Microbiology and Biotechnology
Email: korneev_dv@vector.nsc.ru
Россия, Dolgoprudnyi, Moscow region, 141700; Obolensk, Moscow region, 142279
V. Generalov
Vector State Research Center of Virology and Biotechnology
Email: korneev_dv@vector.nsc.ru
Россия, Kol’tsovo, Novosibirsk region, 630559
B. Zaitsev
Vector State Research Center of Virology and Biotechnology
Email: korneev_dv@vector.nsc.ru
Россия, Kol’tsovo, Novosibirsk region, 630559
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