The Influence of Dinitrosyl Iron Complexes on the Physicochemical Characteristics of Rat Blood Components

Resumo

Abstract—The effect of injected intravenously dinitrosyl iron complexes with glutathione ligand on the structural and conformational characteristics of albumin, which is a primary ligand of these complexes in plasma, and on molecules of the lipid phase in the erythrocyte membrane, which constitute the microenvironment of dinitrosyl iron complexes in erythrocytes, was analyzed. The analysis of EPR spectra of 5- and 16-DOXYL stearic acid spin probes in plasma and erythrocyte suspensions before and after injection of dinitrosyl iron complexes with glutathione was performed in the experiments. It has been found that injection of these complexes into an organism does not affect the molecular motion in albumin hydrophobic regions in plasma and erythrocyte membranes, while at the same time they stimulate incorporation of charged spin labels into the erythrocyte membrane. Apparently, this was due to a change in the charge on the outer surface of the erythrocytes.