Peculiarities of the growth and morphology of human fibroblasts cultured on substrates modified with nanosized fibers of collagen and chitosan

Abstract

The relationship between cell morphology and the topography and composition of a substratecoating material based on nanosized fibers of collagen and chitosan was studied during culturing the cell culture of normal human embryonic fibroblasts. The morphology of cells grown on modified substrates (in particular, the number of cilia and flagella up to 100 nm in diameter) was studied by atomic force microscopy at the submicron level in comparison with their proliferative activity on glass substrates. Methods for controlling the growth activity by changing the structure and composition of the coating material were proposed. The effect of the reduced pH of the coating material surface on growth activity and cell morphology was studied. Methods of spray application of colloidal solutions of biopolymers were proposed.