Email this article Reconstruction of the spatial structure of inulinase from Kluyveromyces marxianus to find regulatory pathways of its catalytic activity
- Authors: Abdullatypov A.V.1, Kondratyev M.S.2, Holyavka M.G.3, Artyukhov V.G.3
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Affiliations:
- Institute of Basic Biological Problems
- Institute of Cell Biophysics
- Voronezh State University
- Issue: Vol 61, No 4 (2016)
- Pages: 565-571
- Section: Molecular Biophysics
- URL: https://ogarev-online.ru/0006-3509/article/view/152038
- DOI: https://doi.org/10.1134/S0006350916040023
- ID: 152038
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Abstract
Reconstruction of the spatial structure of inulinase (EC 3.2.1.7) from Kluyveromyces marxianus (an enzyme that hydrolyzes inulin and other fructose-based polymers to fructose) was carried out by highthroughput computational modeling. A structural model of a closely related homologous protein, viz., invertase from yeast Saccharomyces cerevisiae (PDB-ID: 4EQV), was used as a template. The reconstructed model can be used for computer calculations for optimizing the biotechnological feasibility of inulinase.
About the authors
A. V. Abdullatypov
Institute of Basic Biological Problems
Author for correspondence.
Email: azatik888@yandex.ru
Russian Federation, ul. Institutskaya 2, Pushchino, Moscow oblast, 142290
M. S. Kondratyev
Institute of Cell Biophysics
Email: azatik888@yandex.ru
Russian Federation, ul. Institutskaya 3, Pushchino, Moscow oblast, 142290
M. G. Holyavka
Voronezh State University
Email: azatik888@yandex.ru
Russian Federation, Universitetskaya pl. 1, Voronezh, 394018
V. G. Artyukhov
Voronezh State University
Email: azatik888@yandex.ru
Russian Federation, Universitetskaya pl. 1, Voronezh, 394018
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