Nitric oxide modulation of the crystallogenic properties of a biological fluid

Abstract

A comparative analysis of the influence of different nitric oxide forms on the character of the dehydration structuring of human serum samples was carried out. The effects of an NO-containing gas flow that was generated by a Plazon device (800 and 80 ppm), an experimental NO generator (20, 50, 75, and 100 ppm), as well as by glutathione-containing dinitrosyl iron complexes (3 mM/L) were investigated using 15 healthy donors. The influence of endogenous sodium on serum crystallization of intact and NO treated blood samples was evaluated. It was found that the effect of NO on the crystallogenic properties of blood serum is directly determined by its concentration and form (free or bound), as well as by the presence of reactive oxygen species in a gas flow. The most pronounced stimulatory effect was observed for the bound NO form, dinitrosyl iron complexes with glutathione ligands. Low NO concentrations modulated the crystallogenic properties of blood serum, while the best stimulatory effect was demonstrated by the gas flow that contained 20 ppm nitric oxide. In contrast, high NO concentrations (800 ppm) inhibited the crystallogenic activity of the biological medium due to an increase in the destruction of structural elements by many times, which lead to the formation of an additional band in the marginal zone of the microscope specimen.