The Effect of Polyoxyethylene–Polyoxypropylene Triblock Copolymers on the Loading Degree of Poly-(Lactic-co-Glycolic Acid) Copolymer-Based Microparticles Containing Chlorin e6 and Ethidium Bromide in Mesenchymal Stem Cells

A. A. Temnov; A. N. Sklifas; N. I. Kukushkin; S. P. Krechetov; E. V. Gorina; T. A. Astrelina; D. Yu. Usupzhanova; Yu. B. Suchkova; I. V. Kobzeva; A. S. Samoilov

doi:10.1134/S0006350919020179

The Effect of Polyoxyethylene–Polyoxypropylene Triblock Copolymers on the Loading Degree of Poly-(Lactic-co-Glycolic Acid) Copolymer-Based Microparticles Containing Chlorin e6 and Ethidium Bromide in Mesenchymal Stem Cells

Authors: Temnov A.A.¹^,2^,3, Sklifas A.N.¹, Kukushkin N.I.¹, Krechetov S.P.², Gorina E.V.², Astrelina T.A.³, Usupzhanova D.Y.³, Suchkova Y.B.³, Kobzeva I.V.³, Samoilov A.S.³
Affiliations:
1. Institute of Cell Biophysics, Federal Research Center Pushchino Scientific Center of Biological Investigations, Russian Academy of Sciences
2. Moscow Institute of Physics and Technology (State University)
3. Burnasyan Federal Medical Biophysical Center, Federal Medical Biological Agency of Russia
Issue: Vol 64, No 2 (2019)
Pages: 224-232
Section: Cell Biophysics
URL: https://ogarev-online.ru/0006-3509/article/view/152899
DOI: https://doi.org/10.1134/S0006350919020179
ID: 152899

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Abstract

The effect of different polyoxyethylene–polyoxypropylene triblock copolymers, their concentration, and mode of action on the loading of poly-(lactic-co-glycolic acid) copolymer-based microparticles containing such medicinal agents as radachlorin (chlorin e₆) or ethidium bromide in mesenchymal stem cells was studied. It has been shown that medicinal agents encapsulated inside microparticles affect the loading of these particles in the cytoplasm of mesenchymal stem cells. The number of cells that absorbed the particles with chlorin e₆ is approximately two times lower than that in the experiments with ethidium bromide. It has been shown that pretreatment of microparticles with triblock copolymers is more efficient for loading them in cells compared with simultaneous introduction of triblock copolymers and particles into the culture medium. Treatment of ethidium bromide-containing microparticles with triblock copolymers is not efficient for their loading in mesenchymal stem cells compared to the control. The exception is Pluronic 123; when particles are treated with it at concentrations of 1 and 2%, the loading of particles in cells increases compared to the control by factors of approximately 11 and 5, respectively. For particles with chlorin e₆, their pretreatment with triblock copolymers at a concentration of 4% is most efficient; the loading of the pretreated particles in cells is increased by factors of approximately 3 to 11.

Keywords

mesenchymal stem cells, poly-(lactic-co-glycolic acid) copolymer microparticles, triblock copolymers, adsorption, chlorin e₆, ethidium bromide

About the authors

A. A. Temnov

Institute of Cell Biophysics, Federal Research Center Pushchino Scientific Center of Biological Investigations, Russian Academy of Sciences; Moscow Institute of Physics and Technology (State University); Burnasyan Federal Medical Biophysical Center, Federal Medical Biological Agency of Russia

Email: nikukushkin@mail.ru
Russian Federation, Pushchino, Moscow oblast, 142290; Dolgoprudny, Moscow oblast, 141701; Moscow, 123098

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