A study of the impacts of low-intensity light irradiation in the red (λmax = 635 nm) and green (λmax = 520 nm) ranges on the proliferative activity and gene expression profiles of MNNG/hos cells and human fetal fibroblasts

Abstract

It has been shown that a single low-intensity (0.146 mW/cm²) irradiation of a cell culture of human fetal fibroblasts by a red LED (λ_max = 635 nm) at a dose of 175.2 mJ/cm² led to a slight stimulation of culture growth, whereas light irradiation by a green LED (λ_max = 520 nm) at the same dose did not alter the proliferative activity of cultured fibroblasts. The growth of transformed MNNG/hos cells did not change at the selected irradiation modes. Both types of irradiation changed the expression profile of the proliferation and osteogenic differentiation genes of the fibroblast culture. In the transformed MNNG/hos cells, the red light promoted the expression of stemness genes, osteogenic differentiation genes, and cell-death markers. The transcription activity of cell-death markers and checkpoints in the MNNG/hos cells increased under the influence of the green light.