Electrical Stimulation of Human Connective Tissue Cells on Layers of Composite Structures with a Nanocarbon Framework


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Resumo

A technique for electrical stimulation of growth of connective tissue cells on composite structures with a nanocarbon framework was developed. Cells were stimulated with pulsed electric signal (amplitude, 200 mV; pulse duration, 2.5 ms) for 5 h. A study of the effect of the electrical stimulation on the viability of cells showed an increase in the proliferative activity and in the density of cells adhering to the surface of composite structure layers. The aspect ratio for the cell structure subjected to the electrical stimulation was 1.4 times greater than for nonstimulated cells. The developed technique can be used in tissue engineering, an important branch of regenerative medicine.

Sobre autores

M. Mezentseva

Ivanovsky Institute of Virology, National Research Center for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya

Email: gerasimenko@bms.zone
Rússia, Moscow

L. Russu

Ivanovsky Institute of Virology, National Research Center for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya

Email: gerasimenko@bms.zone
Rússia, Moscow

P. Privalova

National Research University of Electronic Technology (MIET)

Email: gerasimenko@bms.zone
Rússia, Zelenograd, Moscow

A. Gerasimenko

National Research University of Electronic Technology (MIET); I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation

Autor responsável pela correspondência
Email: gerasimenko@bms.zone
Rússia, Zelenograd, Moscow; Moscow

N. Zhurbina

National Research University of Electronic Technology (MIET)

Email: gerasimenko@bms.zone
Rússia, Zelenograd, Moscow

V. Petukhov

National Research University of Electronic Technology (MIET)

Email: gerasimenko@bms.zone
Rússia, Zelenograd, Moscow

E. Pyankov

National Research University of Electronic Technology (MIET)

Email: gerasimenko@bms.zone
Rússia, Zelenograd, Moscow

L. Ichkitidze

National Research University of Electronic Technology (MIET); I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation

Email: gerasimenko@bms.zone
Rússia, Zelenograd, Moscow; Moscow

I. Suetina

Ivanovsky Institute of Virology, National Research Center for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya

Email: gerasimenko@bms.zone
Rússia, Moscow

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