C-fos, ERK1/2, MAP2, NOTCH1 Proteins Expression Patterns in Human Cerebral Cortex Neurons after Ischemic Stroke
- 作者: Sergeeva S.P.1, Lyundup A.V.1, Beregovykh V.V.1, Litvitskiy P.F.1, Savin A.A.2, Gorbacheva L.R.3, Kiseleva E.V.4, Breslavich I.D.3, Kutsenko K.I.5,6, Shishkina L.V.7
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隶属关系:
- I.M. Sechenov First Moscow State Medical University (Sechenov University)
- A.I. Yevdokimov Moscow State University of Medicine and Dentistry
- Lomonosov Moscow State University
- Koltzov Institute of Developmental Biology of Russian Academy of Sciences
- Bureau of Forensic Medicine of Moscow Healthcare Department
- International Legal Institute
- N.N. Burdenko Scientific Research Neurosurgery
- 期: 卷 75, 编号 3 (2020)
- 页面: 226-233
- 栏目: NEUROLOGY AND NEUROSURGERY: CURRENT ISSUES
- URL: https://ogarev-online.ru/vramn/article/view/125693
- DOI: https://doi.org/10.15690/vramn1295
- ID: 125693
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Background. The search for protein (these include c-fos, ERK1/2, MAP2, NOTCH1) expression that provide neuroplasticity mechanisms of the cerebral cortex after ischemic stroke (IS) patterns is an urgent task.
Aims — to reveal c-fos, ERK1/2, MAP2, NOTCH1 proteins expression patterns in human cerebral cortex neurons after IS.
Materials and methods. We studied 9 left middle cerebral artery (LMCA) IS patients cerebral cortex samples from 3 zones: 1 — the zone adjacent to the necrotic tissue focus; 2 — zone remote from the previous one by 4–7 cm; 3 — zone of the contralateral hemisphere, symmetric to the IS focus. Control samples were obtained from 3 accident died people. Identification of targeted proteins NSE, c-fos, ERK1/2, MAP2, NOTCH1 was performed by indirect immunoperoxidase immunohistochemical method.
Results. Moving away from the ischemic focus, there is an increase in the density of neurons and a decrease in the damaged neurons proportion, the largest share of c-fos protein positive neurons in zone 2, NOTCH1 positive neurons in zone 1, smaller fractions of ERK1/2 and MAP2 positive neurons compared to the control only in samples of zone 1.
Conclusions. With the IS development, the contralateral hemisphere is intact tissue increased activation zone, while the zones 1 and 2 have pathological activation signs. In zone 1 of the range, the adaptive response of the tissue decreases, and in zone 2 it expands. Therefore, a key target for therapeutic intervention is zone 2.
作者简介
Svetlana Sergeeva
I.M. Sechenov First Moscow State Medical University (Sechenov University)
编辑信件的主要联系方式.
Email: svetlanapalna@mail.ru
ORCID iD: 0000-0002-0083-1213
SPIN 代码: 4257-9498
MD, PhD
俄罗斯联邦, 8, bld. 2, Trubetskaya str., 119991, MoscowAleksey Lyundup
I.M. Sechenov First Moscow State Medical University (Sechenov University)
Email: lyundup@gmail.com
ORCID iD: 0000-0002-0102-5491
SPIN 代码: 4954-3004
MD, PhD
俄罗斯联邦, MoscowValery Beregovykh
I.M. Sechenov First Moscow State Medical University (Sechenov University)
Email: beregovykh@ramn.ru
ORCID iD: 0000-0002-0210-4570
SPIN 代码: 5940-7554
MD, PhD, Professor, Aсademiсian of the RAS
俄罗斯联邦, MoscowPetr Litvitskiy
I.M. Sechenov First Moscow State Medical University (Sechenov University)
Email: litvicki@mma.ru
ORCID iD: 0000-0003-0151-9114
SPIN 代码: 6657-5937
MD, PhD, Professor, Corresponding Member of the RAS
俄罗斯联邦, MoscowAleksey Savin
A.I. Yevdokimov Moscow State University of Medicine and Dentistry
Email: lasavin@mail.ru
ORCID iD: 0000-0002-6340-8623
SPIN 代码: 9162-6720
MD, PhD, Professor
俄罗斯联邦, MoscowLiubov Gorbacheva
Lomonosov Moscow State University
Email: gorbacheva@mail.bio.msu.ru
ORCID iD: 0000-0002-3910-8831
SPIN 代码: 4394-0575
PhD, Professor
俄罗斯联邦, MoscowEkaterina Kiseleva
Koltzov Institute of Developmental Biology of Russian Academy of Sciences
Email: evkiseleva@mail.ru
ORCID iD: 0000-0002-2270-6595
SPIN 代码: 7421-8496
PhD
俄罗斯联邦, MoscowIlya Breslavich
Lomonosov Moscow State University
Email: br_i@inbox.ru
ORCID iD: 0000-0002-9321-9102
SPIN 代码: 9615-9237
ассистент
俄罗斯联邦, MoscowKirill Kutsenko
Bureau of Forensic Medicine of Moscow Healthcare Department; International Legal Institute
Email: ceamler@gmail.com
ORCID iD: 0000-0001-9299-5153
SPIN 代码: 8964-3717
MD, PhD
俄罗斯联邦, MoscowLyudmila Shishkina
N.N. Burdenko Scientific Research Neurosurgery
Email: lshishkina@nsi.ru
ORCID iD: 0000-0001-7045-7223
SPIN 代码: 6341-2050
MD, PhD
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