Мақаланы E-mail арқылы жіберу Generation of LEPR K nockout Rabbits with CRISPR/CAS9 System
- Авторлар: Silaeva Y.Y.1, Safonova P.D.1, Popov D.V.2, Filatov М.А.1, Okulova Y.D.1, Shafei R.А.3, Skobel О.I.2, Vysotskii D.E.2, Gubarev Y.D.4, Glazko V.I.2, Glazko T.T.2, Georgiev P.G.1, Kosovsky G.Y.2, Shepelev М.V.1
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Мекемелер:
- Institute of Gene Biology Russian academy of sciences
- Afanas`ev Research Institute of Fur – Bearing Animal Breeding and Rabbit Breeding
- Moscow State University
- Belgorod National Research University
- Шығарылым: Том 518, № 1 (2024)
- Беттер: 36-45
- Бөлім: Articles
- URL: https://ogarev-online.ru/2686-7389/article/view/273245
- DOI: https://doi.org/10.31857/S2686738924050071
- ID: 273245
Дәйексөз келтіру
Ашық рұқсат
Рұқсат берілді
Тек жазылушылар үшін
Аннотация
In humans mutations in the LEPR gene, encoding leptin hormone receptor, are associated with the development of morbid obesity, lipid metabolism dysregulation, defects of fertility. In rodents spontaneous mutations in Lepr gene have been described. Lepr knock-out (KO) animals have been generated using classical KO technology or CRISPR/Cas9 system. In rodents lipid metabolism is significantly different from that in humans or rabbits, therefore rabbits are considered as the most relevant model of morbid obesity and lipid metabolism dysregulation in humans, but so far LEPR knock-out rabbits have not been reported. Here using CRISPR/Cas9 system we generated LEPR knock-out rabbit by introducing a deletion of the region around exon 10 of the LEPR gene. We showed that the body weight of the knock-out rabbit was significantly higher than an average body weight of the wild type rabbits. CRISPR/Cas9-mediated technology for the generation of the LEPR knock-out rabbits will allow for the development of the model of morbid obesity and endocrine defects in humans due to leptin receptor mutations.
Негізгі сөздер
Авторлар туралы
Y. Silaeva
Institute of Gene Biology Russian academy of sciences
Хат алмасуға жауапты Автор.
Email: mshepelev@mail.ru
Center for Precision Genome Editing and Genetic Technologies for Biomedicine
Ресей, MoscowP. Safonova
Institute of Gene Biology Russian academy of sciences
Email: mshepelev@mail.ru
Ресей, Moscow
D. Popov
Afanas`ev Research Institute of Fur – Bearing Animal Breeding and Rabbit Breeding
Email: mshepelev@mail.ru
Ресей, Rodniki, Moscow region
М. Filatov
Institute of Gene Biology Russian academy of sciences
Email: mshepelev@mail.ru
Center for Precision Genome Editing and Genetic Technologies for Biomedicine
Ресей, MoscowY. Okulova
Institute of Gene Biology Russian academy of sciences
Email: mshepelev@mail.ru
Center for Precision Genome Editing and Genetic Technologies for Biomedicine
Ресей, MoscowR. Shafei
Moscow State University
Email: mshepelev@mail.ru
Ресей, Moscow
О. Skobel
Afanas`ev Research Institute of Fur – Bearing Animal Breeding and Rabbit Breeding
Email: mshepelev@mail.ru
Ресей, Rodniki, Moscow region
D. Vysotskii
Afanas`ev Research Institute of Fur – Bearing Animal Breeding and Rabbit Breeding
Email: mshepelev@mail.ru
Ресей, Rodniki, Moscow region
Y. Gubarev
Belgorod National Research University
Email: mshepelev@mail.ru
Ресей, Belgorod
V. Glazko
Afanas`ev Research Institute of Fur – Bearing Animal Breeding and Rabbit Breeding
Email: mshepelev@mail.ru
Ресей, Rodniki, Moscow region
T. Glazko
Afanas`ev Research Institute of Fur – Bearing Animal Breeding and Rabbit Breeding
Email: mshepelev@mail.ru
Ресей, Rodniki, Moscow region
P. Georgiev
Institute of Gene Biology Russian academy of sciences
Email: mshepelev@mail.ru
Academician of the RAS
Ресей, MoscowG. Kosovsky
Afanas`ev Research Institute of Fur – Bearing Animal Breeding and Rabbit Breeding
Email: mshepelev@mail.ru
Ресей, Rodniki, Moscow region
М. Shepelev
Institute of Gene Biology Russian academy of sciences
Email: mshepelev@mail.ru
Center for Precision Genome Editing and Genetic Technologies for Biomedicine
Ресей, MoscowӘдебиет тізімі
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