Email this article Expression of MSX1, HOXA11 and TP53I3 in the endometrium associated with the onset of pregnancy after repeated failed IVF attempts in patients with tubo-peritoneal factor infertility
- Authors: Knyazeva E.A.1, Kuznetsova M.V.1, Burmenskaya O.V.1, Donnikov A.E.1, Kalinina E.A.1
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Affiliations:
- Kulakov Research Center for Obstetrics, Gynecology and Perinatology
- Issue: Vol 22, No 1 (2020)
- Pages: 23-28
- Section: ORIGINAL ARTICLE
- URL: https://ogarev-online.ru/2079-5831/article/view/33965
- DOI: https://doi.org/10.26442/20795696.2020.1.200028
- ID: 33965
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Abstract
Relevance. The success of the in vitro fertilisation (IVF) program, among other factors, depends on the readiness of the endometrium to accept the embryo. It is believed that this is possible during the so-called «implantation window», the timing of which can be shifted under the influence of various factors. Evaluation of endometrial receptivity and the «implantation window» based on analysis of endometrial gene expression before embryo transfer is a promising approach for predicting the likelihood of pregnancy in IVF programs.
Aim. To construct a classifier based on the expression of endometrial genes for predicting the outcome of an IVF program in patients with tubal-peritoneal infertility factor and repeated failed IVF attempts in history.
Materials and methods. Before the IVF program, a genome-wide transcriptome profiling of endometrial samples of 15 women with tubal-perioneal infertility factor and repeated unsuccessful IVF attempts in history was carried out using Affymetrix arrays. Potential genes capable of classifying IVF program outcomes were selected, after which the expression of these genes was analyzed by qPCR-RT in the endometrium of 47 women to construct IVF outcome classifiers based on the expression of pairs or triples of genes.
Results. A classifier based on the expression of the triple of genes MSX1 (HOX7), HOXA11, and TP53I3 made it possible to determine the onset of pregnancy in an IVF program with a sensitivity of 73% and a specificity of 71% with an area under the ROC-curve (AUC) of 0.738 (95% confidence interval 0.577–0.898). Earlier, a relationship was found between the expression of these genes and receptivity of the endometrium, which suggests that these genes play a role in the onset of the «implantation window».
Conclusions. The use of a classifier based on the genes MSX1 (HOX7), HOXA11, and TP53I3 can determine the readiness of the endometrium to accept an embryo and create an individual prognosis of the outcome of an IVF program in women with tubal-peritoneal infertility factor and repeated failed IVF attempts in history.
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##article.viewOnOriginalSite##About the authors
Ekaterina A. Knyazeva
Kulakov Research Center for Obstetrics, Gynecology and Perinatology
Author for correspondence.
Email: dr.knyazeva.ea@gmail.com
ORCID iD: 0000-0002-1472-2018
Postgraduate Student
Russian Federation, MoscowMaria V. Kuznetsova
Kulakov Research Center for Obstetrics, Gynecology and Perinatology
Email: mkarja@mail.ru
ORCID iD: 0000-0003-3790-0427
Cand. Sci. (Biol.)
Russian Federation, MoscowOlga V. Burmenskaya
Kulakov Research Center for Obstetrics, Gynecology and Perinatology
Email: o_bourmenskaya@oparina4.ru
D. Sci. (Biol.)
Russian Federation, MoscowAndrey E. Donnikov
Kulakov Research Center for Obstetrics, Gynecology and Perinatology
Email: a_donnikov@oparina4.ru
ORCID iD: 0000-0003-3504-2406
Cand. Sci. (Med.)
Russian Federation, MoscowElena A. Kalinina
Kulakov Research Center for Obstetrics, Gynecology and Perinatology
Email: e_kalinina@oparina4.ru
ORCID iD: 0000-0002-8922-2878
D. Sci. (Med.)
Russian Federation, MoscowReferences
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