Combined effect of polypeptide complexes on the development of organotypic culture of rat tissues
- Authors: Chalisova N.I.1,2, Ryzhak G.A.2, Egozova E.S.3
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Affiliations:
- I.P. Pavlov Institute of Physiology of the Russian Academy of Sciences
- St. Petersburg Institute of Bioregulation and Gerontology
- A.I. Herzen Russian State Pedagogical University
- Issue: Vol 21, No 1 (2023)
- Pages: 61-64
- Section: Original research
- URL: https://ogarev-online.ru/1728-2918/article/view/143917
- DOI: https://doi.org/10.29296/24999490-2023-01-09
- ID: 143917
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Abstract
Introduction. The formation of effective bioregulators for the maintenance of the basic physiological functions, so as polypeptide complexes (PPCs), is one of the actual task of modern biology and medicine.
Purpose of study. Effect of combination of polypeptide complexes (PPCs) from thymus (Thymalin) with PPC from liver (Ventvil), PPC from kidney (Nephropept) and PPC from brain, (Сortexin) on the cellular proliferation of rat corresponding tissues.
Methods. Organotypic tissue culture of rat spleen, liver, kidney and brain cortex under different PPCs and their combinations effect.
Results. All PPCs increase statistically reliable the square index (SI) of rat corresponding tissues explants of young rats skin explants on 20–27%, as compared to the control. There was no potentiate effect and the stimulation of the cellular proliferation was at the account of this PPC, which stimulated the proliferation by its single effect.
Conclusion. The obtained data create a basis for using by the patient combined pathology of two different PPCs, each of them will have effect on the regeneration stimulation only of corresponding tissue.
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##article.viewOnOriginalSite##About the authors
Natalia I. Chalisova
I.P. Pavlov Institute of Physiology of the Russian Academy of Sciences; St. Petersburg Institute of Bioregulation and Gerontology
Author for correspondence.
Email: ni_chalisova@mail.ru
ORCID iD: 0000-0002-2371-0043
SPIN-code: 2139-7608
Expert scientific worker of Pavlov Institute of Physiology, Russian Academy of Sciences, Expert scientific worker of Petersburg Institute of bioregulation and Gerontology. Doctor of biological sciences, Professor.
Russian Federation, nab. Makarova, 6, St. Petersburg, 199034; Аve. Dynamo, 3, St. Petersburg, 197110Galina A. Ryzhak
St. Petersburg Institute of Bioregulation and Gerontology
Email: galina@gerontology.ru
ORCID iD: 0000-0003-2536-1438
Associate Director of Saint Petersburg Institute of Bioregulation and Gerontology in scientific investigation. Doctor of medical sciences, рrofessor.
Russian Federation, Аve. Dynamo, 3, St. Petersburg, 197110Ekaterina S. Egozova
A.I. Herzen Russian State Pedagogical University
Email: ekaterina_egozova@mail.ru
ORCID iD: 0000-0002-0055-3778
magister of Herzen State Pedagogical University of Russia
Russian Federation, Moika River Embankment, 48, Saint Petersburg, 191186References
- Lee A.C., Harris J.L., Khanna K.K., Hong J.H. A Comprehensive Review on Current Advances in Peptide Drug Development and Design. Int. J. Mol. Sci. 2019; 20 (10): 2383. DOI: 10.3390/ ijms20102383
- Owji H., Nezafat N., Negahdaripour M., Hajiebrahimi A., Ghasemi Y. A comprehensive review of signal peptides: Structure, roles, and applications. Eur. J. Cell. Biol. 2018; 97 (6): 422–41. doi: 10.1016/j.ejcb.2018.06.003.
- Khavinson V.Kh. Peptides and ageing. Neuroendocrinology Letters. Special Issue. 2002; 144.
- Хавинсон В.Х., Кузник Б.И., Рыжак Г.А. Пептидные геропротекторы — эпигенетические регуляторы физиологических функций организма. СПб.: РГПУ им. А.И. Герцена. 2014; 271. ISBN 978-5-5-8064-2035-10.
- Khavinson V., Trofimova S., Trofimov A., Solomin I. Molecular Physiological Aspects of Regulatory Eff ect of Peptide Retinoprotectors. Stem. Cell Rev. Reports. 2019; 1–4. DOI: 10.1007/ s12015-019-09882-
- Хавинсон В.Х Лекарственные пептидные препараты: прошлое, настоящее, будущее. Клиническая медицина. .2020; 98 (3): 165–77. https://doi.org/10.30629/0023-2149-202 [Khavinson V.Kh, Lekarstvennie peptidnie preparati: proshloe, nastoyashchee, budushchee. Klinicheskaya medizcina. 2020; 98 ( 3): 165–77. (in Russian)].
- Рыжак Г.А., Чалисова Н. И., Ивко О.М., Заломаева Е.С.,. Иванова П.Н. Протекторное влияние биорегуляторных пептидов на развитие органотипической культуры тканей в присутствии цитостатика. Молекулярная медицина. 2020; 18 (6): 52–6. https://doi. org/10.29296/24999490-2020-06-08 [Rizhak G.A., Chalisova N.I., Ivko O.M., Zalomaeva E.S., Ivanova P.N. Protective effect of bioregulatory peptides on the organotypic tissue culture development in cytostatic presence. Molekulyarnaya meditsina. 2020; 18 (6): 52–56. https://doi. org/10.29296/24999490-2020-06-08/ (in Russian)].
- Кузник Б.И., Рыжак Г.А., Хавинсон В.Х. Полипептидный комплекс сосудов и его роль в регуляции физиологических функций при возрастной патологии. Успехи геронтологии. 2019; 32 (1–2): 174–9. [Kuznik B.I., Rizhak G.A., Khavinson V.Kh, Polipeptidnii complex sosudov I ego rol v regulyacii fiziologicheskikh funkcii pri vozrastnoii patologii. Uspekhi gerontologii. 2019; 32 (1–2): 174–9 (in Russian)].
- Соколова И.Б., Рыжак Г.А., Хавинсон В.Х. Функциональная кумуляция влияния пептидного биорегулятора сосудов на микроциркуляцию в коре головного мозга у спонтанно гипертензивных крыс. Успехи геронтологии. 2017; 30 (5): 671–5. [Sokolova I.B., Rizhak G.A., Khavinson V.Kh. Funkcionalnaya kumulayciya vliyaniya peptidnogo bioregulyatora sosudov na microcirkulyaciyu v kore golovnogo mozga u spontanno gipertenzivnikh kris. Uspekhi gerontologii. 2017; 30 (5): 671–5 (in Russian)].
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